产品名称
IgG Sepharose™ 6 Fast Flow, Cytiva 17-0969-01, pack of 10 mL
ligand
human IgG
packaging
pack of 10 mL
manufacturer/tradename
Cytiva 17-0969-01
storage condition
(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)
matrix
highly cross-linked 6% agarose
average diameter
90 μm
cleaning in place
3-12
working range
3-10
capacity
~3 mg binding capacity(Protein A)
storage temp.
2-8°C
Analysis Note
Features and Benefits
- IgG coupled to the highly cross-linked 6% agarose matrix Sepharose™ 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
- Binds at least 2 mg protein A/mL medium
- Suitable for tandem affinity purification (TAP) of protein complexes
General description
lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
实验方案
This page shows how to purify protein A-tagged proteins using Cytiva products.
This page shows how to prepare samples for purification with affinity chromatography.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
相关内容
利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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