ligand
Cibacron Blue
包装
pack of 50 mL
制造商/商品名称
Cytiva 17-0948-01
储存条件
(20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)
基质
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
3-13
working range
4-12
适用性
suitable for bioprocess medium
储存温度
2-8°C
相关类别
一般描述
Blue Sepharose™ 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Blue Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.
The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
Blue Sepharose™ 6 Fast Flow has been developed and tested in co-operation with leading large scale manufacturers and is used in routine commercial production.
Blue Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Blue Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特点和优势
- Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
- BioProcess medium supported for industrial applications and well-established in approved processes
- Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity
储存及稳定性
分析说明
法律信息
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
实验方案
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.
Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.
This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.
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