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保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 25 mL
制造商/商品名称
Cytiva 17-0921-07
基质
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
working range
3-12
容量
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
适用性
suitable for bioprocess medium
相关类别
一般描述
应用
IMAC Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose™ 6 Fast FLow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target Protein.
The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.
IMAC Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support
特点和优势
- Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
- Convenient purification of histidine-tagged Proteins, also when Ni2+ is not the best choice of metal ion
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in the convenient and time-saving prepacked HiTrap® and HiPrep™ formats.
储存及稳定性
分析说明
法律信息
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
商品
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
实验方案
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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