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安全信息

GE17-0906-01

NHS Act Sepharose™ 4 Fast Flow

Cytiva 17-0906-01, pack of 25 mL

别名:

NHS-Activated Sepharose FF, pre-activated agarose matrix

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

ligand

N-hydroxysuccinimide activated groups

包装

pack of 25 mL

制造商/商品名称

Cytiva 17-0906-01

储存条件

(100% Isopropanol)

基质

4% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

3-13(Ligand dependent)

ligand dependent

3-13

工作范围

3-12(3 (ligand dependent)-12 (ligand dependent))

适用性

suitable for bioprocess medium

储存温度

2-8°C

一般描述

NHS-activated Sepharose™ 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides.

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.

This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.

NHS-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特点和优势

  • NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation gives a high degree of substitution of the selected ligand
  • NHS coupling method results in chemically stable ligand attachment
  • BioProcess medium supported for industrial applications

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Exclamation mark
GHS07

警示用语：

Danger

危险声明

H225,H319,H336

储存分类代码

3 - Flammable liquids

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

色谱柱填充和抗体亲和层析的制备

本页介绍了高效的色谱柱填充和抗体亲和层析的制备。

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

实验方案

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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