产品名称
Protein A-Sepharose™ CL-4B, Cytiva 17-0780-01, pack of 1.5 g
ligand
native protein A
crosslinking
cross-linked
packaging
pack of 1.5 g
manufacturer/tradename
Cytiva 17-0780-01
storage condition
(20% Ehtanol)
parameter
150 cm/hr flow rate at 25 °C
matrix
4% cross-linked agarose
matrix active group
functional group
particle size
45-165 μm
average diameter
90 μm
cleaning
2-10
pH
3—9
working range
3-9
capacity
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
separation technique
affinity
storage temp.
2-8°C
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Features and Benefits
- Well documented for immunoglobulin purification and fractionation
- Strong binding to Fc region of IgG
- High capacity - 20 mg human IgG/mL.
- Suited for immunoprecipitation procedures
General description
Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
Sepharose is a trademark of Cytiva
存储类别
13 - Non Combustible Solids
法规信息
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