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ligand
native protein A
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 1.5 g
制造商/商品名称
Cytiva 17-0780-01
储存条件
(20% Ehtanol)
基质
4% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-9
容量
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
储存温度
2-8°C
相关类别
一般描述
Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
特点和优势
- Well documented for immunoglobulin purification and fractionation
- Strong binding to Fc region of IgG
- High capacity - 20 mg human IgG/mL.
- Suited for immunoprecipitation procedures
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Sepharose is a trademark of Cytiva
法规信息
新产品
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