产品名称
Glutathione Sepharose™ 4B, Cytiva 17-0756-04, pack of 300 mL
packaging
pack of 300 mL
manufacturer/tradename
Cytiva 17-0756-04
storage condition
(20% ethanol)
parameter
<100 cm/hr flow rate (sample loading)
100-300 cm/hr flow rate (wash and elution)
technique(s)
liquid chromatography (LC): suitable
matrix
4% agarose
matrix active group
Glutathione
average diameter
90 μm
cleaning in place
4-13
working range
4-13
capacity
>25 mg binding capacity (recombinant GST / ml)
separation technique
affinity
shipped in
ambient
storage temp.
2-30°C
Analysis Note
Features and Benefits
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
General description
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
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