产品名称
DEAE Sepharose™ Fast Flow, Cytiva 17-0709-01, pack of 500 mL
ligand
diethylaminoethyl
description
Ion Exchanger Type (value)
packaging
pack of 500 mL
manufacturer/tradename
Cytiva 17-0709-01
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
2-12
suitability
suitable for bioprocess medium
Analysis Note
Features and Benefits
- Well-proven weak anion exchanger developed for industrial downstream processes
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
General description
DEAE Sepharose Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. DEAE Sepharose Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with DEAE Sepharose Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.
As member of the BioProcess media range, DEAE Sepharose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Preparation Note
Legal Information
signalword
Danger
hcodes
Hazard Classifications
Flam. Liq. 2
存储类别
3 - Flammable liquids
wgk
WGK 1
法规信息
商品
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
实验方案
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
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