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安全信息

GE17-0640-01

Act Thiol Sepharose™ 4B

Cytiva 17-0640-01, pack of 15 g

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

包装

pack of 15 g

制造商/商品名称

Cytiva 17-0640-01

基质

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

2-8

工作范围

2-8

储存温度

2-8°C

一般描述

Activated Thiol Sepharose™ 4B medium is a medium used for reversible immobilization of molecules containing thiol groups under mild conditions. Optimized for immobilization of large molecules.

Activated Thiol Sepharose™ 4B is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBr activated Sepharose™ 4B. Activated Thiol Sepharose™ 4B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for immobilizing thiol containing biomolecules.

特点和优势

  • Reversible coupling of proteins and large biomolecules with thiol groups to Sepharose™ 4B via a glutathione spacer arm
  • The ligand is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBractivated Sepharose™ 4B.
  • Well suited for covalent chromatography of large molecules such as enzymes and nucleic acids
  • Gel also reacts with heavy metal ions and with alkyl and aryl halides. Addition reactions occur with compounds containing C=O, C=C, and N=N bonds
  • Separate thiol-containing proteins from non-thiol-containing proteins

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

储存分类代码

13 - Non Combustible Solids

法规信息

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