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Merck
CN

GE17-0568-01

Benzamidine-Sepharose™ 6B

Cytiva 17-0568-01

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ligand

p-aminobenzamidine

manufacturer/tradename

Cytiva 17-0568-01

matrix

6% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-13

working range

3-11

capacity

13 mg binding capacity(trypsin/ml drained media)

storage temp.

2-8°C

storage condition

(20% Ethanol)

General description

Benzamidine Sepharose™ 6B with immobilized p-aminobenzamidine (pABA) is used for removal and/or purification of serine poteases (e.g. thrombin).

Application

Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose™ 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine Sepharose™ 6B and can thus be used for purification and/or removal of these substances. Trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein, collagenase and clostripain are some of the serine proteases that have been purified on Benzamidine Sepharose™ 6B. For recombinant purification, Benzamidine Sepharose™ 6B can be used for removal of serine proteases such as thrombin and enterokinase after cleavage of purification tags

For recombinant purification, Benzamidine Sepharose™ 6B can be used for removal of serine proteases such as thrombin and enterokinase after cleavage of purification tags

Features and Benefits

  • For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
  • Sepharose™ 6B, is 6% cross-linked Agarose medium.
  • Established medium for purifying serine proteases, e.g. trypsin or thrombin.
  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

存储类别

10 - Combustible liquids

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