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安全信息

GE17-0529-01

Calmodulin-Sepharose™ 4B

Cytiva 17-0529-01, pack of 10 mL

别名:

Calmodulin Affinity Resin, Calmodulin-Sepharose

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

ligand

calmodulin

包装

pack of 10 mL

制造商/商品名称

Cytiva 17-0529-01

储存条件

(20% Ehtanol)

基质

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

4-9

工作范围

4-9

储存温度

2-8°C

一般描述

Calmodulin Sepharose™ 4B affinity media for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.

Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca2+ complexes are particularly stable.

特点和优势

  • For single-step purification of native calmodulin-binding proteins
  • Suitable for tandem affinity purification (TAP) of protein complexes
  • Purification of calmodulin-regulated proteins from all eukaryotic cells

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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Mahmoud Hajj Chehade et al.
Cell chemical biology, 26(4), 482-492 (2019-01-29)
Ubiquinone (UQ) is a polyprenylated lipid that is conserved from bacteria to humans and is crucial to cellular respiration. How the cell orchestrates the efficient synthesis of UQ, which involves the modification of extremely hydrophobic substrates by multiple sequential enzymes

商品

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

Performing a Purification with Calmodulin Sepharose 4B

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from Cytiva.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

用于亲和层析的色谱柱填充和制备实验方案

该页面展示了如何使用Cytiva的Tricorn™或XK色谱柱进行亲和层析色谱柱填充和制备。

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

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相关内容

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

蛋白质Pull-Down技术

利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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