产品名称
Calmodulin-Sepharose™ 4B, Cytiva 17-0529-01, pack of 10 mL
ligand
calmodulin
packaging
pack of 10 mL
manufacturer/tradename
Cytiva 17-0529-01
storage condition
(20% Ehtanol)
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
4-9
working range
4-9
storage temp.
2-8°C
相关类别
Analysis Note
Features and Benefits
- For single-step purification of native calmodulin-binding proteins
- Suitable for tandem affinity purification (TAP) of protein complexes
- Purification of calmodulin-regulated proteins from all eukaryotic cells
General description
Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca2+ complexes are particularly stable.
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
实验方案
This page shows how to prepare samples for purification with affinity chromatography.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from Cytiva.
相关内容
利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持