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主要文件

COO原产地证书/COA分析证书

安全信息

安全和法规

GE17-0413-01

HiTrap^® Blue High Performance

Name: HiTrap<SUP>®</SUP> Blue High Performance
Brand: SIGMA

Cytiva 17-0413-01, pack of 1 × 5 mL

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About This Item

UNSPSC代码:

23151816

NACRES:

NA.56

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ligand

Cibacron Blue F3G-A

包装

pack of 1 × 5 mL

制造商/商品名称

Cytiva 17-0413-01

参数

42 psi

柱床尺寸

16 mm × 25 mm

柱床体积

5 mL

色谱柱内径

16 mm

尺寸

平均零件尺寸

34 μm

cleaning

3-13

工作范围

4-12

一般描述

HiTrap^® Blue HP is prepacked with Blue Sepharose^™ High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins.

应用

Blue Sepharose^™ High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some Proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.

特点和优势

Can be used for depletion of albumin from serum.
Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked Agarose medium and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning.
The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.