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ligand
native protein A
包装
pack of 5 × 5 mL
制造商/商品名称
Cytiva 17-0403-03
储存条件
(20% Ethanol)
参数
42 psi
柱床尺寸
16 mm × 25 mm
柱床体积
5 mL
色谱柱内径
16 mm
尺寸
平均零件尺寸
34 μm
cleaning
2-10
工作范围
3-9
储存温度
2-8°C
一般描述
HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient formulation.
特点和优势
- High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
- 34 μm bead size for high-performance, high-resolution purifications.
- Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
- Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
- Robust, easy-to-use method.
储存及稳定性
分析说明
其他说明
法律信息
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
实验方案
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
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