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安全信息

GE17-0403-03

HiTrap® Protein A High Performance

Cytiva 17-0403-03, pack of 5 × 5 mL

别名:

HiTrap Protein A HP, high performance column, antibody purification

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About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

native protein A

包装

pack of 5 × 5 mL

制造商/商品名称

Cytiva 17-0403-03

储存条件

(20% Ethanol)

参数

42 psi

柱床尺寸

16 mm × 25 mm

柱床体积

5 mL

色谱柱内径

16 mm

尺寸

平均零件尺寸

34 μm

cleaning

2-10

工作范围

3-9

储存温度

2-8°C

一般描述

HiTrap® Protein A HP is a prepacked Protein A Sepharose High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient formulation.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特点和优势

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids

法规信息

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商品

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

实验方案

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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