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安全信息

GE17-0403-01

HiTrap® Protein A High Performance

Cytiva 17-0403-01, pack of 1 × 5 mL

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UNSPSC代码:
23151816
NACRES:
NA.56

ligand

native protein A

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包装

pack of 1 × 5 mL

制造商/商品名称

Cytiva 17-0403-01

储存条件

(20% Ethanol)

参数

flow rate
<20 mL/min flow rate
42 psi

柱床尺寸

16 mm × 25 mm

柱床体积

5 mL

色谱柱内径

16 mm

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning

2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range

3-9

储存温度

2-8°C

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相关类别

一般描述

HiTrap® Protein A HP is a prepacked Protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.

特点和优势

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

法规信息

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Esteban Cruz et al.
Pharmaceutics, 13(11) (2021-11-28)
Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives. Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity

商品

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

实验方案

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from Cytiva.

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

线性流速（cm/小时）与体积流速（mL/min)之间的换算

本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

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