GE17-0402-01

HiTrap^® Protein A High Performance

Name: HiTrap<SUP>®</SUP> Protein A High Performance
Brand: SIGMA

Cytiva 17-0402-01, pack of 5 × 1 mL

别名:

Protein A Affinity Column

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关于此项目

NACRES:

NA.56

UNSPSC Code:

23151816

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产品名称

HiTrap^® Protein A High Performance, Cytiva 17-0402-01, pack of 5 × 1 mL

form

prepacked column

crosslinking

cross-linked

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-0402-01

storage condition

(20% Ethanol)

parameter

0.5 Mpa max. pressure
1 mL/min flow rate
4 mL/min max. flow rate

technique(s)

liquid chromatography (LC): suitable

bed volume

1 mL

column I.D. × L

7 mm × 25 mm

matrix

highly cross-linked agarose, spherical

matrix active group

Protein A

avg. part. size

34 μm

cleaning

2-10

working range

3-9

capacity

~20 mg binding capacity (human IgG / mL resin)

separation technique

affinity

storage temp.

2-8°C

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

FPLC is a trademark of Cytiva

General description

HiTrap^® Protein A HP is a prepacked Protein A Sepharose^™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap^® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
34 μm bead size for high-performance, high-resolution purifications.
Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC^™ System.
Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
Robust, easy-to-use method.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.