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安全信息

GE17-0034-01

Sephadex® G-25 Coarse

Cytiva 17-0034-01, pack of 100 g

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About This Item

UNSPSC代码:
23151817
NACRES:
NA.56

包装

pack of 100 g

制造商/商品名称

Cytiva 17-0034-01

储存条件

dry media

基质

cross-linked dextran

粒径

>100 μm (dry)

cleaning in place

2-13

工作范围

2-13

适用性

suitable for bioprocess medium

一般描述

Sephadex® G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

应用

Sephadex® is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).

Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.

特点和优势

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Excellent recovery and minimum sample dilution
  • Available in prepacked HiPrep™ Desalting and HiTrap® Desalting columns for fast and convenient desalting
  • BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

储存分类代码

13 - Non Combustible Solids

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

Fractional Precipitation Protocol for Affinity Chromatography Samples

This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

亲和层析缓冲液置换和脱盐

如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

相关内容

Sephadex® and Darcy’s Law

Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography

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