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ligand
alkali-stabilized protein A-derived (E. coli)
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 5 × 1 mL
制造商/商品名称
Cytiva 11-0034-93
储存条件
(20% Ehtanol)
(20% Ethanol)
参数
flow rate
<4 ml/min flow rate
42 psi
柱床尺寸
7 mm × 25 mm
柱床体积
1 mL
色谱柱内径
7 mm
基质
highly cross-linked agarose
平均零件尺寸
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-13.7(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-12
容量
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
适用性
suitable for bioprocess medium
储存温度
2-8°C
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相关类别
一般描述
HiTrap® MabSelect™ columns are prepacked with MabSelect™ a bioprocess medium for capuring of Mabs from large sample volumes.
特点和优势
- Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
- Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.
分析说明
其他说明
法律信息
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
实验方案
This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from Cytiva.
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