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安全和法规

GE11-0033-99

His GraviTrap^™

Name: His GraviTrap<SUP>™</SUP>
Brand: SIGMA

Cytiva 11-0033-99

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UNSPSC代码:

12161501

NACRES:

NA.32

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保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包装

pkg of 10 ea

制造商/商品名称

Cytiva 11-0033-99

柱床体积

1 mL

色谱柱内径

15 mm

基质

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14(Ni²⁺-stripped medium)

working range

3-12(Ni²⁺-stripped medium.)

容量

~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)
~40 mg binding capacity(histidine-tagged protein)

适用性

suitable for bioprocess medium

一般描述

His GraviTrap^™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose^™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap^™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.

特点和优势

For fast and simple purification of histidine-tagged* proteins.
Convenient, prepacked gravity-flow column.
Allows direct purification of both clarified and unclarified cell lysates.
High binding capacity; approximately 40 mg of pure protein per column.
Compatible with a wide range of reducing agents, detergents, denaturants and other additives.