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Merck
CN

G9891

Sigma-Aldrich

β-甘油磷酸盐 二钠盐 水合物

powder, BioReagent, suitable for cell culture

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别名:
BGP, 甘油2-磷酸酯 二钠盐 水合物
线性分子式:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
分子量:
216.04 (anhydrous basis)
EC 号:
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:

产品线

BioReagent

形式

powder

技术

cell culture | mammalian: suitable

杂质

≤0.20% α-isomer

mp

102-104 °C (lit.)

SMILES字符串

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

InChI key

ROPZSVKNEIIIDE-UHFFFAOYSA-L

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应用

β-甘油磷酸盐是激酶反应缓冲液中丝氨酸-苏氨酸磷酸酶的一种经典抑制剂。BGP通常与其他磷酸酶/蛋白酶抑制剂组合,以实现广谱抑制。它可用作有机磷酸盐供体,并且已用于使间充质干细胞分化为成骨细胞型细胞的培养基。BGP也用于缓冲乳酸菌的M17培养基,以进行重组蛋白表达。
对嵌入天然胶原/壳聚糖凝胶(用于组织工程)中的骨髓衍生干细胞进行诱导分化。
Glycero-2-phosphate is a component of dexamethasone based hormone cocktail used to induce mesechymal stem cell (MSC) differentiation into osteoblasts.

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

新产品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is this Glycerol 2-phosphate disodium salt hydrate, Product G9891, a cGMP Product?

    Yes. This product is produced under cGMP.

  6. What can I use to solubilize Glycerol 2-phosphate disodium salt hydrate, Product G9891?

    This product is soluble in water at a concentration of 50 mg/mL, yielding a clear, colorless solution.

  7. What concentration of Glycerol 2-phosphate disodium salt hydrate, Product G9891, is used in cell culture?

    Product No. G9891 is tested in cell culture for toxic effects at 0.1 mg/mL. It is used at 10-20 mM for inhibition of phosphatase activity.

  8. Is there an alternative product if Glycerol 2-phosphate disodium salt hydrate, Product G9891, is on backorder?

    Similar products are Product Nos. 50020, G6376 and G1150.

  9. Glycerol 2-phosphate disodium salt hydrate, Product G9891, is "cell culture tested". What does that mean for this product?

    Growth studies are carried through two subculture generations at 0.1 mg/mL. Cells are counted, and growth is plotted as a logarithmic function of time in culture. Seeding efficiencies, doubling time, and final cell densities are determined. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity.

  10. What is the solution stability of Glycerol 2-phosphate disodium salt hydrate, Product G9891?

    β-Glycerophosphate undergoes very slow hydrolysis at 100°C in 1 M hydrochloric acid. The implication is that solutions would be relatively stable at acidic pH.

  11. What is the water content in Glycerol 2-phosphate disodium salt hydrate, Product G9891?

    The water content can vary from lot to lot.  It is typically in the range of 24-31%.  The information can be found on the lot-specific certificate of analysis.

  12. What is Glycerol 2-phosphate disodium salt hydrate, Product G9891, used for?

    β-glycerophosphate is used in cell culture as an inhibitor of alkaline phosphatase. It is routinely used in culture of osteoblast/osteoclast cell lines where the activity of alkaline phosphatase needs to be inhibited (measurement of phosphorylated proteins, etc). A search on PubMed lists over 2500 articles citing "glycerophosphate" and "culture". Most references are for induction of bone lineage cells or for inhibition of phosphatase activity in other types of cell culture.

  13. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Hemophilia is an X-linked bleeding disorder, and patients with hemophilia are deficient in a biologically active coagulation factor. This study was designed to combine the efficiency of lentiviral vector transduction techniques with murine adipose tissue-derived stem/stromal cells (mADSCs) as a
Wen-Ning Xu et al.
Frontiers in endocrinology, 10, 922-922 (2020-02-13)
Long-term and high dose glucocorticoid treatment can cause decreased viability and function of osteoblasts, which leads to osteoporosis and osteonecrosis. In this study, we investigated the role and mechanism of action of HIF-1α in glucocorticoid-induced osteogenic inhibition in MC3T3-E1 cells.
Mario-Luca Suvà et al.
Cancer research, 69(5), 1776-1781 (2009-02-12)
Cancer stem cells that display tumor-initiating properties have recently been identified in several distinct types of malignancies, holding promise for more effective therapeutic strategies. However, evidence of such cells in sarcomas, which include some of the most aggressive and therapy-resistant
Monica Echeverry-Rendon et al.
Colloids and surfaces. B, Biointerfaces, 193, 111153-111153 (2020-06-07)
Magnesium (Mg) is a material widely used in industrial applications due to its low weight, ductility, and excellent mechanical properties. For non-permanent implants, Mg is particularly well-suited because of its biodegradability, while its degradation products are not harmful. However, Mg
Nastaran Abbarin et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(5), 775-785 (2014-11-20)
Amelotin (AMTN) is a recently discovered protein that is specifically expressed during the maturation stage of dental enamel formation. It is localized at the interface between the enamel surface and the apical surface of ameloblasts. AMTN knock-out mice have hypomineralized

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