G8880
谷氨酰胺酶 来源于大肠杆菌
Grade V, lyophilized powder, ≥50 units/mg protein
别名:
L-谷氨酰胺酰胺水解酶
产品名称
谷氨酰胺酶 来源于大肠杆菌, Grade V, lyophilized powder, ≥50 units/mg protein
biological source
Escherichia coli
type
Grade V
form
lyophilized powder
mol wt
110 kDa
composition
Protein, ~30% biuret
Quality Level
specific activity
≥50 units/mg protein
solubility
water: 1 mg/mL (Clear w/Particle to Sl. Hazy w/Particles)
cation traces
NH4+: <0.1 μg/unit
foreign activity
NADH Oxidase ≤0.01%
storage temp.
−20°C
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Application
来自大肠杆菌的谷氨酰胺酶已被用于：
- 测定大脑皮层组织提取物中的谷氨酰胺
- 固定在氨基丙基甲硅烷基化控制孔玻璃（CPG）上用于测定谷氨酰胺
- 与体外黑色素瘤细胞、MCF-7和OAW-42细胞一同用于细胞增殖和侵袭性检测。
Biochem/physiol Actions
来自大肠杆菌的谷氨酰胺酶与谷氨酰胺类似物阿西维奇一起可调节癌细胞的增殖和侵袭性。通过降低肿瘤负担，细菌谷氨酰胺酶与癌症患者的寿命延长相关。
谷氨酰胺酶可催化谷氨酰胺向谷氨酸的转化。
General description
谷氨酰胺酶是一种酰胺水解酶。它在N端具有催化结构域，而C端延伸具有STAS结构域。
Other Notes
在37°C、pH 4.9条件下，一单位每分钟可水解1.0 μmole的L-谷氨酰胺。
Physical form
含有琥珀酸钾和EDTA的冻干粉
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Porta-caval shunting changes neuronal sensitivity to ammonia
Raabe W and Onstad G
Journal of the Neurological Sciences, 71(2-3), 307-314 (1985)
Acivicin with glutaminase regulates proliferation and invasion of human MCF-7 and OAW-42 cells-An in vitro study
Roy S, et al.
Indian Journal of Experimental Biology, 46, 22-26 (2008)
Ayşegül Erdem et al.
Nature communications, 13(1), 2013-2013 (2022-04-21)
Metabolic programs can differ substantially across genetically distinct subtypes of acute myeloid leukemia (AML). These programs are not static entities but can change swiftly as a consequence of extracellular changes or in response to pathway-inhibiting drugs. Here, we uncover that
Selective flow injection procedures for the determination of nitrogen containing analytes by gasdialytic-fluorimetric detection of enzymatically generated ammonia
Mana H and Spohn U
Analytica Chimica Acta, 325(1-2), 93-104 (1996)
Modulation of metastatic potential of B16F10 melanoma cells by acivicin: synergistic action of glutaminase and potentiation of cisplatin cytotoxicity
Roy S and Maity P
Asian Pacific Journal of Cancer Prevention, 8(2), 301-301 (2007)
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