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Merck
CN

G8420

β-葡萄糖醛酸酶 来源于大肠杆菌

≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

别名:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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关于此项目

化学文摘社编号:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
MFCD00199013
EC 号:
3.2.1.31 (BRENDA, IUBMB)

主要文件

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产品名称

β-葡萄糖醛酸酶 来源于大肠杆菌, ≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

recombinant

expressed in E. coli overproducing strain

form

lyophilized powder

specific activity

≥20,000 units/mg protein

mol wt

290 kDa

composition

, ~25%

storage temp.

−20°C

Quality Level

300

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相关类别

Analysis Note

含有缓冲盐和稳定剂。

Application

比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据，关于使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。
用于尿液代谢物分析中葡糖苷酸结合物的水解

Other Notes

一个Sigma或改良的Fishman单位将在37℃，pH6.8（30分钟测定）下每小时从酚酞葡糖苷酸中释放1.0μg酚酞。

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000436125
0000436125
0000252708
0000250210
0000235771

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Enzymes
Hans Ulrich Bergmeyer
Methods of Analysis, 2, 460-461 (1974)
V Graef et al.
Clinical chemistry, 23(3), 532-535 (1977-03-01)
We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded
Xiaoyan Liu et al.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 42(1), 67-74 (2013-03-19)
To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP
Regina Goetz et al.
Nature reviews. Molecular cell biology, 14(3), 166-180 (2013-02-14)
Fibroblast growth factors (FGFs) mediate a broad range of functions in both the developing and adult organism. The accumulated wealth of structural information on the FGF signalling pathway has begun to unveil the underlying molecular mechanisms that modulate this system
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商品

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

实验方案

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

相关内容

Data Sheet - G8420

Instructions

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