质量
含有缓冲盐和稳定剂。
单位定义
一个Sigma或改良的Fishman单元将在37℃,pH6.8(30分钟测定)下每小时从酚酞葡糖苷酸中释放1.0 μg酚酞。
警示用语:
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
RSC advances, 10(39), 22966-22971 (2020-06-16)
UDP-glucuronosyltransferases (UGTs) and β-glucuronidase (GUS) catalyze entirely distinct metabolism reactions. UGTs are responsible for the glucuronidation of a variety of drugs, endogenous and environmental chemicals, whereas GUS hydrolyzes glucuronides and liberates the parent substrates. Information on the overlap of ligand
Enzymes
Methods of Enzymatic Analysis, 2, 460-461 (1974)
Frontiers in chemistry, 9, 709581-709581 (2021-08-03)
For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform
The Journal of nutrition, 151(7), 1802-1816 (2021-04-21)
Curcuminoids from turmeric rhizome have significant health benefits but low bioavailability. To assess the pharmacokinetics of a novel natural turmeric dried colloidal suspension compared with 4 other turmeric formulations (including a standardized extract) at their respective recommended dosages. Thirty healthy men
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine.
Health Laboratory Science, 50, 286-289 (2004)
商品
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.
实验方案
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
为了使用β-葡糖醛酸酶优化水解,必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估,例如孵育时间、温度、水解pH、酶源和酶浓度。
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
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