G8255
谷丙转氨酶 来源于猪心脏
lyophilized powder, ≥75 units/mg protein
别名:
L-丙氨酸:2-酮戊二酸氨基转移酶, GPT, 丙氨酸氨基转移酶（催化浓度）
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关于此项目
化学文摘社编号:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-561-4
MDL number:
Beilstein/REAXYS Number:
3562140
Specific activity:
≥75 units/mg protein
Biological source:
Porcine heart
biological source
Porcine heart
form
lyophilized powder
specific activity
≥75 units/mg protein
composition
Protein, 75-95%
technique(s)
cell culture | mammalian: suitable
color
light yellow to yellow
foreign activity
L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%, glutamic-oxalacetic transaminase ≤0.1%
storage temp.
−20°C
Quality Level
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General description
谷丙转氨酶（GPT）主要分布于肝脏。
研究领域：细胞信号传导
研究领域：细胞信号传导
Application
猪心谷丙转氨酶已用于：
- 检测其对人工合成的3-甲基-2-酮戊二酸外消旋混合物进行转氨反应的能力
- 去除人胚肾细胞(HEK293)培养基中的谷氨酸
- 研究乳酸脱氢酶(LD)-丙氨酸转氨酶(ALT)酶偶联反应系统的动力学
Biochem/physiol Actions
谷丙转氨酶 (GPT) 存在于体内大多数细胞中，但在肝脏中浓度最高。由于在肝细胞坏死后 GPT 释放入血液，可作为肝脏疾病的标志物。
谷丙转氨酶（GPT）介导氨基从 l-丙氨酸转移到 α-酮戊二酸，从而产生丙酮酸和谷氨酸。因此，GPT 在糖异生和氨基酸代谢中发挥着关键作用。报道显示多种肿瘤中都有GPT参与。
Physical form
含柠檬酸盐和醋酸盐的冻干粉
Preparation Note
密闭。干燥。锁存或存放在只有合格或授权人员才能进入的区域
Analysis Note
双缩脲法测定蛋白质
Other Notes
一个单位在-37°C、pH 7.6且L-丙氨酸存在条件下将 1.0μmol 酮戊二酸转化成 L -谷氨酸。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin
Chen M, et al.
Royal Society of Chemistry Advances, 5, 103557-103562 (2015)
A Comprehensive Guide to Toxicology in Nonclinical Drug Development
Faqi A S
Biomarkers journal., 2017 null
Giordano Pula et al.
Journal of biochemical and biophysical methods, 64(3), 167-181 (2005-08-23)
The surface expression of G protein-coupled receptors is regulated by internalization. For many receptors, a constitutive level of internalization in the absence of agonist has been reported. The constitutive internalization of metabotropic glutamate receptor 1a (mGluR1a) has been described, but
Wei Guo et al.
Cancers, 12(7) (2020-07-15)
Metabolic reprogramming is an essential hallmark of cancer. Besides the "Warburg effect", cancer cells also actively reprogram amino acid metabolism to satisfy high nutritional demands in a nutrient-poor environment. In the glucose-alanine cycle, exogenous alanine taken up by hepatocytes is
Christoph Mahlert et al.
Journal of the American Chemical Society, 129(39), 12011-12018 (2007-09-06)
The acidic lipopeptides, including the calcium-dependent antibiotics (CDA), daptomycin, and A54145, are important macrocyclic peptide natural products produced by Streptomyces species. All three compounds contain a 3-methyl glutamate (3-MeGlu) as the penultimate C-terminal residue, which is important for bioactivity. Here
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