G8255
谷丙转氨酶 来源于猪心脏
lyophilized powder, ≥75 units/mg protein
别名:
L-丙氨酸:2-酮戊二酸氨基转移酶, GPT, 丙氨酸氨基转移酶（催化浓度）
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关于此项目
化学文摘社编号:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-561-4
MDL number:
Beilstein/REAXYS Number:
3562140
产品名称
谷丙转氨酶 来源于猪心脏, lyophilized powder, ≥75 units/mg protein
biological source
Porcine heart
form
lyophilized powder
specific activity
≥75 units/mg protein
composition
Protein, 75-95%
technique(s)
cell culture | mammalian: suitable
color
light yellow to yellow
foreign activity
L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.1%
storage temp.
−20°C
Quality Level
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Biochem/physiol Actions
谷丙转氨酶 (GPT) 存在于体内大多数细胞中，但在肝脏中浓度最高。由于在肝细胞坏死后 GPT 释放入血液，可作为肝脏疾病的标志物。
谷丙转氨酶（GPT）介导氨基从 l-丙氨酸转移到 α-酮戊二酸，从而产生丙酮酸和谷氨酸。因此，GPT 在糖异生和氨基酸代谢中发挥着关键作用。报道显示多种肿瘤中都有GPT参与。
Analysis Note
双缩脲法测定蛋白质
Application
猪心谷丙转氨酶已用于：
- 检测其对人工合成的3-甲基-2-酮戊二酸外消旋混合物进行转氨反应的能力
- 去除人胚肾细胞(HEK293)培养基中的谷氨酸
- 研究乳酸脱氢酶(LD)-丙氨酸转氨酶(ALT)酶偶联反应系统的动力学
General description
谷丙转氨酶（GPT）主要分布于肝脏。
研究领域：细胞信号传导
研究领域：细胞信号传导
Other Notes
一个单位在-37°C、pH 7.6且L-丙氨酸存在条件下将 1.0μmol 酮戊二酸转化成 L -谷氨酸。
Physical form
含柠檬酸盐和醋酸盐的冻干粉
Preparation Note
密闭。干燥。锁存或存放在只有合格或授权人员才能进入的区域
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Giordano Pula et al.
Journal of biochemical and biophysical methods, 64(3), 167-181 (2005-08-23)
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A Comprehensive Guide to Toxicology in Nonclinical Drug Development
Faqi A S
Biomarkers journal., 2017 null
A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin
Chen M, et al.
Royal Society of Chemistry Advances, 5, 103557-103562 (2015)
Christoph Mahlert et al.
Journal of the American Chemical Society, 129(39), 12011-12018 (2007-09-06)
The acidic lipopeptides, including the calcium-dependent antibiotics (CDA), daptomycin, and A54145, are important macrocyclic peptide natural products produced by Streptomyces species. All three compounds contain a 3-methyl glutamate (3-MeGlu) as the penultimate C-terminal residue, which is important for bioactivity. Here
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Cancers, 12(7) (2020-07-15)
Metabolic reprogramming is an essential hallmark of cancer. Besides the "Warburg effect", cancer cells also actively reprogram amino acid metabolism to satisfy high nutritional demands in a nutrient-poor environment. In the glucose-alanine cycle, exogenous alanine taken up by hepatocytes is
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