G8021
Monoclonal Anti-β-Galactosidase antibody produced in mouse
clone GAL-13, ascites fluid
别名:
Monoclonal Anti-β-Galactosidase
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关于此项目
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
GAL-13, monoclonal
Application:
DB, ELISA (i), ICC
Citations:
31
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GAL-13, monoclonal
contains
15 mM sodium azide
species reactivity
bacteria
technique(s)
dot blot: suitable using soluble β-galactosidase, immunocytochemistry: suitable, indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
Escherichia coli ... lacZ(1036228), lacZ(1036228)
Escherichia coli K12 ... Lacz(945006)
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General description
Monoclonal Anti-β-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β −galactosidase is a E coli protein and is a tetramer with 1023 amino acids.
Immunogen
β-galactosidase from E. coli
Application
Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in:
- immunostaining and histochemistry
- immunostaining and microscopy
- immunohistochemistry
- immunofluorescence staining
- enzyme linked immunosorbent assay(ELISA)
- immuno dot blot
Biochem/physiol Actions
β −galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-β-d-galactopyranoside (oNPG) or p-nitrophenyl-β-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal β -galactosidase anti- β -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background.
The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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