推荐产品
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
GAL-13, monoclonal
包含
15 mM sodium azide
种属反应性
bacteria
技术
dot blot: suitable using soluble β-galactosidase
immunocytochemistry: suitable
indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase
同位素/亚型
IgG1
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此商品 | A7554 | B6804 | P2419 |
|---|---|---|---|
| Gene Information Escherichia coli ... lacZ(1036228), lacZ(1036228) | Gene Information - | Gene Information - | Gene Information - |
| species reactivity bacteria | species reactivity bovine (non-reactive to IgM) | species reactivity bacteria | species reactivity horseradish |
| conjugate unconjugated | conjugate alkaline phosphatase conjugate | conjugate alkaline phosphatase conjugate | conjugate unconjugated |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone GAL-13, monoclonal | clone BG-18, monoclonal | clone BAP-77, monoclonal | clone P6-38, monoclonal |
一般描述
Monoclonal Anti-β-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β −galactosidase is a E coli protein and is a tetramer with 1023 amino acids.
特异性
The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.
免疫原
β-galactosidase from E. coli
应用
Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in:
- immunostaining and histochemistry
- immunostaining and microscopy[1]
- immunohistochemistry
- immunofluorescence staining
- enzyme linked immunosorbent assay(ELISA)
- immuno dot blot
生化/生理作用
β −galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-β-d-galactopyranoside (oNPG) or p-nitrophenyl-β-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal β -galactosidase anti- β -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Thyroid hormone signaling in the mouse retina
Arbogast P, et al.
PLoS ONE, 11(12), e0168003-e0168003 (2016)
Expression of CD34 and Myf5 defines the majority of quiescent adult skeletal muscle satellite cells.
J R Beauchamp et al.
The Journal of cell biology, 151(6), 1221-1234 (2000-12-21)
Skeletal muscle is one of a several adult post-mitotic tissues that retain the capacity to regenerate. This relies on a population of quiescent precursors, termed satellite cells. Here we describe two novel markers of quiescent satellite cells: CD34, an established
Heng Zhao et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 24(6), 681-692 (2004-06-08)
Apoptosis plays a critical role in many neurologic diseases, including stroke. Cytochrome c release and activation of various caspases are known to occur after focal and global ischemia. However, recent reports indicate that caspase-independent pathways may also be involved in
Shuang Li et al.
Science signaling, 7(332), ra62-ra62 (2014-07-06)
Hedgehog (Hh) is a secreted glycoprotein that binds its receptor Patched to activate the G protein (heterotrimeric guanine nucleotide-binding protein)-coupled receptor-like protein Smoothened (Smo). In Drosophila, protein kinase A (PKA) phosphorylates and activates Smo in cells stimulated with Hh. In
Expanding the utility of beta-galactosidase complementation: piece by piece
Broome AM, et al.
Molecular Pharmaceutics, 7(1), 60-74 (2009)
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