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一般描述
半乳糖氧化酶是一种细胞外含铜酶,由半知菌真菌 树状指孢霉分泌。它可催化一系列伯醇(包括D-半乳糖)氧化成相应的醛,同时将氧还原成过氧化氢。
应用
半乳糖氧化酶可作为一种分析工具,用于特异性测定血浆、植物提取物和磷脂中的D-半乳糖。它可用于表征几种聚合物中的末端D-半乳糖苷单元。
生化/生理作用
半乳糖氧化酶可催化D-半乳糖向D-半乳糖-己二醛的转化。
2-脱氧-D-半乳糖、乳糖、甘露糖、棉子糖和水苏糖可与过氧化物酶:邻甲苯胺系统中的半乳糖氧化酶发生反应。
基本上没有观察到D-葡萄糖、L-半乳糖、L-阿拉伯糖或D-葡萄糖醛酸盐的氧化。
2-脱氧-D-半乳糖、乳糖、甘露糖、棉子糖和水苏糖可与过氧化物酶:邻甲苯胺系统中的半乳糖氧化酶发生反应。
基本上没有观察到D-葡萄糖、L-半乳糖、L-阿拉伯糖或D-葡萄糖醛酸盐的氧化。
单位定义
在pH6.0、25℃条件下,一个单位可在过氧化物酶和邻联甲苯胺系统中每分钟产生δ A425=1.0。反应体积=3.4mL,光路= 1cm。
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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