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Merck
CN

G7163

Sigma-Aldrich

α-半乳糖苷酶,位置特异性 来源于大肠杆菌

recombinant, expressed in E. coli, buffered aqueous solution

别名:

1,6-alpha-D-galactoside galactohydrolase, alpha-Galactosidase, melibiase

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.32

重组

expressed in E. coli

质量水平

表单

buffered aqueous solution

比活

≥20 units/mg protein

分子量

80 kDa

运输

wet ice

储存温度

2-8°C

基因信息

Escherichia coli CFT073 ... melA(1037886)

生化/生理作用

从复杂的碳水化合物和糖蛋白中裂解 α(1→3)-和 α(1→6)-连接的非还原性末端半乳糖。在 pH 必须为中性或更高的条件下(例如,活细胞),这种方法对于去除 α-连接的半乳糖特别有效。

单位定义

在 pH 6.5,25°C 下,一个单位每分钟将水解 1 μ 摩尔对硝基苯基 α-D-吡喃半乳糖苷。

外形

该产品是无菌过滤的水性缓冲溶液。

抑制剂

产品编号
说明
价格

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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K Schmid et al.
European journal of biochemistry, 67(1), 95-104 (1976-08-01)
The utilization by Escherichia coli K12 of raffinose as sole carbon source depends on a new raffinose transport system, an invertase and an alpha-galactosidase specified by the Raf-plasmid D1021. The alpha-galactosidase was purified to homogeneity from a mutant strain with
Shuo Gao et al.
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For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform
Delayed anaphylactic reaction to mammalian meat.
K Sekiya et al.
Journal of investigational allergology & clinical immunology, 22(6), 446-447 (2012-10-30)
K Wyatt et al.
Health technology assessment (Winchester, England), 16(39), 1-543 (2012-10-24)
To determine natural history and estimate effectiveness and cost of enzyme replacement therapy (ERT) and substrate replacement therapy (SRT) for patients with Gaucher disease, Fabry disease, mucopolysaccharidosis type I (MPS I), mucopolysaccharidosis type II (MPS II), Pompe disease and Niemann-Pick
Xiao-Liang Pan et al.
The journal of physical chemistry. B, 117(2), 484-489 (2012-12-20)
The enzyme α-galactosidase (α-GAL), a member of glycoside hydrolase family 27, catalyzes the removal of a nonreducing terminal α-galactose residue from polysaccharides, glycolipids, and glycopeptides. α-GAL is believed to have the double displacement retaining reaction mechanism. In this work, the

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