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Merck
CN

G7141

葡萄糖氧化酶 来源于黑曲霉

greener alternative

Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)

别名:

β-D-葡萄糖：氧 1-氧化还原酶, G.Od., GOx

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关于此项目

化学文摘社编号:
9001-37-0
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-601-0
MDL number:
MFCD00063989
EC 号:
1.1.3.4 (BRENDA, IUBMB)

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产品名称

葡萄糖氧化酶 来源于黑曲霉, Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)

SMILES string

O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O

InChI key

WQZGKKKJIJFFOK-VFUOTHLCSA-N

InChI

1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1

type

Type X-S

form

lyophilized powder

specific activity

100,000-250,000 units/g solid (without added oxygen)

mol wt

160 kDa

composition

Protein, ≥65%

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

application(s)

diagnostic assay manufacturing

foreign activity

Catalase ≤5 units/mg protein

greener alternative category

Enabling,

storage temp.

−20°C

Quality Level

200

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Analysis Note

双缩脲法测定的蛋白
可能含有痕量的淀粉酶、麦芽糖酶、糖原酶、转化酶和半乳糖氧化酶。

Application

来自黑曲霉的葡萄糖氧化酶已被用于：
  • 通过葡萄糖氧化酶（GO）法测量葡萄糖含量的（葡萄糖氧化酶）GO试剂
  • 激活人肾癌细胞系以建立氧化应激模型
  • 研究其在糊剂中对生物电极分析性能的影响

葡萄糖氧化酶广泛用于食品和制药工业，以及葡萄糖生物传感器的主要组分。

Biochem/physiol Actions

葡萄糖氧化酶催化β-d-葡萄糖氧化成 d-葡糖酸-β-内酯和过氧化氢，分子氧作为电子受体。
葡萄糖氧化酶（GOX）可将D-葡萄糖氧化为D-葡萄糖酸内酯和过氧化氢。过氧化氢是GOX的催化产物，可用作抗菌和抗真菌剂。它是可安全用于多种工业应用，如焙烤、干蛋粉生产、葡萄酒生产、葡萄糖酸生产等。它具有电化学活性，因此在葡萄糖传感器和燃料电池应用中极为重要。

General description

分子量：160 kDa（凝胶过滤）
pI: 1865
消光系数：E1% = 16.7 (280 nm)

来自黑曲霉的葡萄糖氧化酶是由 2 个相等的亚基组成的二聚体，每个亚基的分子量为 80kDa。每个亚基含有一个黄素腺嘌呤二核苷酸部分和一个铁。该酶是含有 ~16％ 中性糖和 2％ 氨基糖的糖蛋白。该酶还含有 3 个半胱氨酸残基和 8 个 N-连接糖基化的潜在位点。

葡萄糖氧化酶能够以不同的速率氧化 D-己醛糖、单脱氧-D-葡萄糖和甲基-D-葡萄糖。

葡萄糖氧化酶的最佳 pH 为 5.5，虽然它的活性范围很宽，pH 4-7葡萄糖氧化酶对β-D-葡萄糖具有特异性，KM为33-110mM。

葡萄糖氧化酶不需要任何活化剂，但它被Ag+、Hg2+、Cu2+、醋酸苯汞和对-氯甲基苯甲酸酯抑制。它不受非金属 SH 试剂的抑制：N-乙基马来酰亚胺、碘乙酸盐和碘乙酰胺。

葡萄糖氧化酶可用于酶法测定溶液中的 D-葡萄糖。由于葡萄糖氧化酶将β-D-葡萄糖氧化成D-葡糖酸内酯和过氧化氢，因此辣根过氧化物酶通常用作葡萄糖测定的偶联酶。尽管葡萄糖氧化酶对β-D-葡萄糖具有特异性，但是可以对D-葡萄糖溶液进行定量，因为当酶促反应消耗β-D-葡萄糖时，α-D-glucose葡萄糖将变为β-D-葡萄糖。
我们致力于为您带来更加绿色的替代产品，这些产品遵守一项或多项绿色化学12项原则。当用于燃料电池研究时，该产品经优化可提高能效和防止浪费。有关详细信息，请参阅 《Biofiles》的文章。
葡萄糖氧化酶（GOX）是一种黄素蛋白，其质量范围约为130至175kDa。一些真菌和昆虫能够生产GOX。

Other Notes

在 pH 5.1，35℃ 下，一个单位每分钟将1.0 μmole的β-D-葡萄糖氧化成 D-葡糖酸内酯和 H2O2，相当于每分钟吸收22.4 μl O2。如果反应混合物被氧气饱和，则活性最多可增加 100％。

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000502271
0000502274
0000502273
0000502270
0000502270

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What buffer is used during the preparation of Glucose oxidase, Product G7141?

    Glucose oxidase contains potassium phosphate and tris acetate salts (which were present in the lyophilization buffer).

  4. Is Glucose oxidase, Product G7141, inhibited by sodium azide, phenol, heparin, citrate or EDTA?

    Glucose oxidase is not inhibited by sodium azide (1.5 mM), low concentrations of phenol (10 mM), heparin, citrate or EDTA.

  5. How should solutions of Glucose oxidase, Product G7141, be stored?

    No solution stability studies have been performed by Sigma.  However, we would expect solutions prepared in buffer in the pH range of 5-7 to be stable at 2-8°C for at least one week.  For longer term storage, solutions can be stored as frozen aliquots at -20°C (only one freeze/thaw cycle) for at least 6 months.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Glucose oxidase-An overview
Biotechnology Advances, 27(4), 489-501 (2009)
Glucose biosensors based on the immobilization of copper oxide and glucose oxidase within a carbon paste matrix
Luque GL, et al.
Talanta, 66(2), 467-471 (2005)
Interspecies Interactions Determine the Impact of the Gut Microbiota on Nutrient Allocation in Drosophila melanogaster
Newell PD, et al.
Applied and Environmental Microbiology, 80(2), 788-796 (2014)
Glucose oxidase: natural occurrence, function, properties and industrial applications
Wong CM, et al.
Applied Microbiology and Biotechnology, 78(6), 927-938 (2008)
Ewa Szczęsna et al.
European journal of cell biology, 95(12), 521-530 (2016-09-10)
End-binding proteins are capable of tracking the plus-ends of growing microtubules (MTs). The motor protein Ncd, a member of the kinesin-14 family, interacts with EB1 protein and becomes a non-autonomous tip-tracker. Here, we attempted to find out whether at least
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实验方案

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

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