推荐产品
类型
Type X-S
质量水平
形式
lyophilized powder
比活
100,000-250,000 units/g solid (without added oxygen)
分子量
160 kDa
组成
Protein, ≥65%
环保替代产品特性
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
应用
diagnostic assay manufacturing
异质活性
Catalase ≤5 units/mg protein
环保替代产品分类
储存温度
−20°C
InChI
1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1
InChI key
WQZGKKKJIJFFOK-VFUOTHLCSA-N
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相关类别
一般描述
pI: 1865
消光系数:E1% = 16.7 (280 nm)
来自黑曲霉的葡萄糖氧化酶是由 2 个相等的亚基组成的二聚体,每个亚基的分子量为 80kDa。每个亚基含有一个黄素腺嘌呤二核苷酸部分和一个铁。该酶是含有 ~16% 中性糖和 2% 氨基糖的糖蛋白。该酶还含有 3 个半胱氨酸残基和 8 个 N-连接糖基化的潜在位点。
葡萄糖氧化酶能够以不同的速率氧化 D-己醛糖、单脱氧-D-葡萄糖和甲基-D-葡萄糖。
葡萄糖氧化酶的最佳 pH 为 5.5,虽然它的活性范围很宽,pH 4-7葡萄糖氧化酶对β-D-葡萄糖具有特异性,KM为33-110mM。
葡萄糖氧化酶不需要任何活化剂,但它被Ag+、Hg2+、Cu2+、醋酸苯汞和对-氯甲基苯甲酸酯抑制。它不受非金属 SH 试剂的抑制:N-乙基马来酰亚胺、碘乙酸盐和碘乙酰胺。
葡萄糖氧化酶可用于酶法测定溶液中的 D-葡萄糖。由于葡萄糖氧化酶将β-D-葡萄糖氧化成D-葡糖酸内酯和过氧化氢,因此辣根过氧化物酶通常用作葡萄糖测定的偶联酶。尽管葡萄糖氧化酶对β-D-葡萄糖具有特异性,但是可以对D-葡萄糖溶液进行定量,因为当酶促反应消耗β-D-葡萄糖时,α-D-glucose葡萄糖将变为β-D-葡萄糖。
应用
- 通过葡萄糖氧化酶(GO)法测量葡萄糖含量的(葡萄糖氧化酶)GO试剂
- 激活人肾癌细胞系以建立氧化应激模型
- 研究其在糊剂中对生物电极分析性能的影响
生化/生理作用
质量
单位定义
分析说明
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What buffer is used during the preparation of Glucose oxidase, Product G7141?
Glucose oxidase contains potassium phosphate and tris acetate salts (which were present in the lyophilization buffer).
Is Glucose oxidase, Product G7141, inhibited by sodium azide, phenol, heparin, citrate or EDTA?
Glucose oxidase is not inhibited by sodium azide (1.5 mM), low concentrations of phenol (10 mM), heparin, citrate or EDTA.
How should solutions of Glucose oxidase, Product G7141, be stored?
No solution stability studies have been performed by Sigma. However, we would expect solutions prepared in buffer in the pH range of 5-7 to be stable at 2-8°C for at least one week. For longer term storage, solutions can be stored as frozen aliquots at -20°C (only one freeze/thaw cycle) for at least 6 months.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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实验方案
Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.
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