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G6920

内-β-半乳糖苷酶 from Bacteroides fragilis

Name: 内-β-半乳糖苷酶 from <I>Bacteroides fragilis</I>
Brand: SIGMA

recombinant, expressed in E. coli, ≥140 units/mg protein, buffered aqueous solution

别名:

β-半乳糖苷细菌, 角蛋白酶

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关于此项目

UNSPSC Code:

12352204

NACRES:

NA.54

EC 号:

3.2.1.103 (BRENDA, IUBMB)

MDL number:

MFCD00131433

Specific activity:

≥140 units/mg protein

Recombinant:

expressed in E. coli

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属性

recombinant

expressed in E. coli

Quality Level

100

conjugate

(Glucosaminoglycan)

sterility

aseptically filled

form

buffered aqueous solution

specific activity

≥140 units/mg protein

mol wt

32 kDa

storage temp.

2-8°C

说明

Application

内-β-半乳糖苷酶已用于分级蛋白质分离。它可用于人内皮细胞内皮分泌组的糖蛋白质组中的去糖基化。

Biochem/physiol Actions

未分枝的重复聚乙酰乳糖胺中的内部β(1-4)半乳糖连接[GlcNAc β(1-3)Gal β (1-4)] 结构是优选的底物。

诸如硫酸角质素的硫酸化结构也会被裂解。底物的支化或岩藻糖基化可减少或完全抑制裂解。半乳糖上C-6的硫酸化将阻断裂解。取决于与优选底物的偏差，新乳酸基团的低聚糖会以极低的速率而被裂解。例如，Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc会以硫酸角质素5X10^-5的速率而被裂解

β-半乳糖苷酶可将乳糖切割成单糖成分、葡萄糖和半乳糖。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物异乳糖。

Physical form

20 mM Tris-HCl，pH 7.5中的无菌填充溶液

Other Notes

在pH 5.8、37 °C条件下，一单位酶每分钟可从牛角质角蛋白硫酸盐中释放1.0 μ摩尔的还原性糖。

wgk

WGK 1

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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全球贸易项目编号

货号	GTIN
G6920-.5UN	04061833642238