G6920
内-β-半乳糖苷酶 from Bacteroides fragilis
recombinant, expressed in E. coli, ≥140 units/mg protein, buffered aqueous solution
别名:
β-半乳糖苷 细菌, 角蛋白酶
产品名称
内-β-半乳糖苷酶 from Bacteroides fragilis, recombinant, expressed in E. coli, ≥140 units/mg protein, buffered aqueous solution
recombinant
expressed in E. coli
conjugate
(Glucosaminoglycan)
sterility
aseptically filled
form
buffered aqueous solution
specific activity
≥140 units/mg protein
mol wt
32 kDa
storage temp.
2-8°C
Quality Level
Application
内-β-半乳糖苷酶已用于分级蛋白质分离。它可用于人内皮细胞内皮分泌组的糖蛋白质组中的去糖基化。
Biochem/physiol Actions
未分枝的重复聚乙酰乳糖胺中的内部β(1-4)半乳糖连接[GlcNAc β(1-3)Gal β (1-4)] 结构是优选的底物。
诸如硫酸角质素的硫酸化结构也会被裂解。底物的支化或岩藻糖基化可减少或完全抑制裂解。半乳糖上C-6的硫酸化将阻断裂解。取决于与优选底物的偏差，新乳酸基团的低聚糖会以极低的速率而被裂解。例如，Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc会以硫酸角质素5X10-5的速率而被裂解
β-半乳糖苷酶可将乳糖切割成单糖成分、葡萄糖和半乳糖。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物异乳糖。
诸如硫酸角质素的硫酸化结构也会被裂解。底物的支化或岩藻糖基化可减少或完全抑制裂解。半乳糖上C-6的硫酸化将阻断裂解。取决于与优选底物的偏差，新乳酸基团的低聚糖会以极低的速率而被裂解。例如，Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc会以硫酸角质素5X10-5的速率而被裂解
β-半乳糖苷酶可将乳糖切割成单糖成分、葡萄糖和半乳糖。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物异乳糖。
Other Notes
在pH 5.8、37 °C条件下，一单位酶每分钟可从牛角质角蛋白硫酸盐中释放1.0 μ摩尔的还原性糖。
Physical form
20 mM Tris-HCl，pH 7.5中的无菌填充溶液
wgk
WGK 1
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Maria Vistnes et al.
PloS one, 9(3), e89621-e89621 (2014-03-07)
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Xiaoke Yin et al.
Molecular & cellular proteomics : MCP, 12(4), 956-978 (2013-01-25)
Previous proteomics studies have partially unraveled the complexity of endothelial protein secretion but have not investigated glycosylation, a key modification of secreted and membrane proteins for cell communication. In this study, human umbilical vein endothelial cells were kept in serum-free
William Mark Erwin et al.
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Salvatore Santamaria et al.
Scientific reports, 9(1), 10914-10914 (2019-07-31)
ADAMTS (A Disintegrin-like and Metalloproteinase domain with Thrombospondin type 1 Motif)-1, -4 and -5 share the abilities to cleave large aggregating proteoglycans including versican and aggrecan. These activities are highly relevant to cardiovascular disease and osteoarthritis and during development. Here
Salvatore Santamaria et al.
Scientific reports, 11(1), 949-949 (2021-01-15)
ADAMTS-5 is a major protease involved in the turnover of proteoglycans such as aggrecan and versican. Dysregulated aggrecanase activity of ADAMTS-5 has been directly linked to the etiology of osteoarthritis (OA). For this reason, ADAMTS-5 is a pharmaceutical target for
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