产品名称
抗-GroEL 兔抗, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
E. coli
technique(s)
indirect ELISA: 1:40,000-1:80,000
western blot: 1:80,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
Escherichia coli ... groEL(913705), groEL(913705)
Escherichia coli K12 ... Grol(948665)
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Application
兔抗GroEL抗体可用作犬恶丝虫、线虫的组织学和免疫组化以及HeLa细胞的蛋白质印迹分析的阳性对照。
Biochem/physiol Actions
GroEL在ATP存在的情况下，与GroES一起协助蛋白质折叠。将醛脱氢酶与GroEL/GroES共表达促进蛋白质折叠并生成更多的可溶性蛋白质。GroES和GroEL结合到蛋白质底物上，将其包裹在一个笼子状的折叠结构中。靶蛋白在折叠完成后从笼子中解离，并伴随三磷酸腺苷（ATP）水解成二磷酸腺苷（ADP）。
该抗体不会与 GroES 和牛 HSP 70 发生交叉反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
GroEL 蛋白属于折叠伴侣蛋白家族。它是一个 58.3 kDa 的蛋白质，具有顶端、中间和赤道功能域。
Immunogen
大肠杆菌中纯化的重组 GroEL。
Physical form
0.01 M 磷酸盐缓冲液（pH 7.4，含有 15 mM 叠氮化钠）溶液
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Daniela Dimastrogiovanni et al.
eLife, 3 (2015-01-01)
Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region
Functional characterization of an archaeal GroEL/GroES chaperonin system Significance of substrate encapsulation.
Figueiredo L, et al.
The Journal of Biological Chemistry, 279(2), 1090-1099 (2004)
Verena Kohler et al.
Nucleic acids research, 46(17), 9201-9219 (2018-07-31)
The dissemination of multi-resistant bacteria represents an enormous burden on modern healthcare. Plasmid-borne conjugative transfer is the most prevalent mechanism, requiring a type IV secretion system that enables bacteria to spread beneficial traits, such as resistance to last-line antibiotics, among
Crystal Structure of Escherichia coli GroEL in Substrate and ADP Unloaded State.
Meena S and Saxena AK
Journal of Physical Chemistry & Biophysics, 6(222), 2161-0398 (2016)
The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol.
Wu X, et al.
BMC Microbiology, 11(1), 87-87 (2011)
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