形式
lyophilized powder
质量水平
比活
25-75 units/mg protein
分子量
~128 kDa (by gel filtration)
组成
Protein, ≥60% biuret
储存温度
−20°C
一般描述
Research area: Cell Signaling
Glycerol kinase (GK) is part of the FGGY carbohydrate kinase family.
Glycerol kinase (GK) is part of the FGGY carbohydrate kinase family.
应用
Glycerokinase from Cellulomonas sp. has been used:
- for determining the kinetic characteristics of human and trypanosomatid phosphofructokinases using an enzyme-linked kinetic assay.
- to study the effect of sugar in fluorescence emission.
- in 2-Arachidonoylglycerol-based fluorescence assay for DH-463, a fluorescent activity-based probe for monoacylglycerol lipase.
生化/生理作用
甘油激酶催化甘油的tge MgATP依赖性磷酸化以产生sn-甘油-3-磷酸，并且是甘油利用中的限速酶。 它也受到果糖-1，6-二磷酸的反馈调节。
物理属性
等电点：4.2
米氏常数：4.4 x 10-5M（甘油），4.3 x 10-4M（ATP）
抑制剂：对对氯汞苯甲酸，重金属离子（Pb++，Fe++，Hg++，Ag+）
最佳pH：9.8（G-3-PDH系统），7.8（G-3-P氧化酶系统）最佳温度：500C
pH稳定性：pH 5.5 x 10.0 (25oC, 20hr)
热稳定性：低于40oC（pH 7.5，15min）
底物特异性：这种酶催化ATP的磷酰基部分末端的立体特异性转移
到甘油的一个伯羟基，
形成sn-甘油-3-P。酶具有最高的
甘油特异性，也能磷酸化二羟基丙酮
和甘油醛（表1，2）。Mg++ 基本上是反应必需的。
.
米氏常数：4.4 x 10-5M（甘油），4.3 x 10-4M（ATP）
抑制剂：对对氯汞苯甲酸，重金属离子（Pb++，Fe++，Hg++，Ag+）
最佳pH：9.8（G-3-PDH系统），7.8（G-3-P氧化酶系统）最佳温度：500C
pH稳定性：pH 5.5 x 10.0 (25oC, 20hr)
热稳定性：低于40oC（pH 7.5，15min）
底物特异性：这种酶催化ATP的磷酰基部分末端的立体特异性转移
到甘油的一个伯羟基，
形成sn-甘油-3-P。酶具有最高的
甘油特异性，也能磷酸化二羟基丙酮
和甘油醛（表1，2）。Mg++ 基本上是反应必需的。
.
单位定义
一个单位为在pH 9.8和25℃下，在PK/LDH的偶联体系内每分钟将1.0 μmole甘油和ATP转化为L-α甘油磷酸盐和ADP。
外形
冻干粉含磷酸盐缓冲盐和葡萄糖酸钠
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Peter M Fernandes et al.
The Biochemical journal, 476(2), 179-191 (2018-11-09)
Eukaryotic ATP-dependent phosphofructokinases (PFKs) are often considered unidirectional enzymes catalysing the transfer of a phospho moiety from ATP to fructose 6-phosphate to produce ADP and fructose 1,6-bisphosphate. The reverse reaction is not generally considered to occur under normal conditions and
Fei Ying et al.
Scientific reports, 14(1), 3922-3922 (2024-02-17)
The influence of lipid metabolism on tumorigenesis and progression has garnered significant attention. However, the role of Glycerol Kinase (GK), a key enzyme in glycerol metabolism, in Esophageal Carcinoma (ESCA) remains unclear. To further elucidate the relationship between GK and
Zhongya Qin et al.
Biomedical optics express, 9(7), 3373-3390 (2018-07-10)
The femtosecond laser ablation in biological tissue produces highly fluorescent compounds that are of great significance for intrinsically labelling ablated tissue in vivo and achieving imaging-guided laser microsurgery. In this study, we analyzed the molecular structures of femtosecond laser-ablated tissues
N Zwaig et al.
Science (New York, N.Y.), 153(3737), 755-757 (1966-08-12)
Fructose-1 ,6-diphosphate is a feedback inhibitor of the catabolic enzyme, glycerol kinase, in Escherichia coli. A mutant was isolated which produced a desensitized enzyme. Glucose was no longer as effective in preventing the utilization of exogenous glycerol by cells which
Purification and properties of glycerol kinase from Escherichia coli.
S I Hayashi et al.
The Journal of biological chemistry, 242(5), 1030-1035 (1967-03-10)
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