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一般描述
β-半乳糖苷酶是由四个相同亚基组成的四聚体，每个亚基为135,000 Da。它是一种含巯基的酶，每个亚基含有19个半胱氨酸残基。
应用
β-半乳糖苷酶与特异性识别目标分子的抗体偶联（酶联免疫检测或 EIA）。 β-半乳糖苷酶还被用作监测启动子基因表达水平的报告酶。
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
β-半乳糖苷酶将乳糖切割成单糖成分，即葡萄糖和半乳糖。它还在反馈环中催化葡萄糖的转糖基化，生成β-半乳糖苷酶的诱导物——异乳糖。
物理属性
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
单位定义
在 37 °C，pH 7.3 条件下，一个单位每分钟可将 1.0μmole 邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和 D-半乳糖。
外形
部分纯化；含有Tris缓冲盐、氯化镁、DL-二硫苏糖醇和 2-巯基乙醇
底物
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
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