一般描述
由Gamborg等描述的Gamborg′B-5培养基可以完全培养基(G5893)进行购买或通过从Vitamin 1000x(G1019)和Basal Salt混合物(G5768)等化学品储备物进行重悬而得。 使用Gamborg′B-5(B5)培养基用于植物细胞系和组织的体外培养,如墨西哥红豆杉、大壶藓、白亚麻、赤桉。
应用
Gamborg′s B-5基础盐混合物已被用于:
- 制备TEX缓冲液的组分
- 作为培养基维持马铃薯悬浮及愈伤组织培养
- 作为主茎节变形方法的培养基
Gamborg′B-5培养基已被用于培养番荔枝愈伤组织。
公式变量
数量
配制成每升培养基含 3.1 克粉末。
警示用语:
Warning
危险声明
危险分类
Ox. Sol. 3
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
危险化学品
动植物来源培养基
Differential induction of redox sensitive extracellular phenolic amides in potato
Physiological and Molecular Plant Pathology, 73(4-5), 109-115 (2008)
Plant, cell & environment, 39(2), 393-415 (2015-08-25)
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus
Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
Bio-protocol, 8(13) (2018)
A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings
In Vitro Cellular & Developmental Biology. Plant, 43(6), 536-549 (2007)
Journal of Asian natural products research, 4(3), 171-174 (2002-07-18)
Callus cultures of Annona squamosa were induced using different explants including petals, seed contents (megagametophyte and embryo) and fruits (mesocarp). Growth of the calli induced from the explants was found to be influenced by the type, concentration and ratio of
商品
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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