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一般描述
β-半乳糖苷酶是一个四聚体，由四个相同的亚基组成，每个亚基的分子量都为135,000。它是一种含巯基的酶，每个亚基含有19个半胱氨酸残基。
应用
β-半乳糖苷酶可与特异性识别靶分子的抗体偶联（酶免疫测定或EISA）。β-半乳糖苷酶也可用作报告酶以监测启动子的基因表达水平。它可以用作具有抗β-半乳糖苷酶抗体的阳性对照蛋白。
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
物理属性
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
单位定义
在37 °C，pH 7.3条件下，一个单位每分钟可将1.0μmole邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和D-半乳糖。
外形
含有Tris缓冲盐和氯化镁
底物
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Alan Merk et al.
IUCrJ, 7(Pt 4), 639-643 (2020-07-23)
We report the determination of the structure of Escherichia coli β-galactosidase at a resolution of ∼1.8 Å using data collected on a 200 kV CRYO ARM microscope equipped with a K3 direct electron detector. The data were collected in a single 24 h
K Kato et al.
Journal of immunology (Baltimore, Md. : 1950), 116(6), 1554-1560 (1976-06-01)
1. A method for the conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from Escherichia coli is described. The method consists of two main steps: treatment of the Fab' fragments containing sulfhydryl groups with excess N,N'-o-phenylenedimaleimide, to introduce
Raimond B G Ravelli et al.
Nature communications, 11(1), 2563-2563 (2020-05-24)
The increasing demand for cryo-electron microscopy (cryo-EM) reveals drawbacks in current sample preparation protocols, such as sample waste and lack of reproducibility. Here, we present several technical developments that provide efficient sample preparation for cryo-EM studies. Pin printing substantially reduces
PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
G R CRAVEN et al.
The Journal of biological chemistry, 240, 2468-2477 (1965-06-01)
Assays for bacterial mucin-desulfating sulfatases.
A M Roberton et al.
Methods in molecular biology (Clifton, N.J.), 125, 417-426 (2000-05-23)
商品
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
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