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The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

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主要文件

安全信息

G5635

Sigma-Aldrich

β 半乳糖苷酶 来源于大肠杆菌

Grade VIII, lyophilized powder, ≥500 units/mg protein

别名:

β-D-半乳糖苷半乳糖水解酶, 乳糖酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

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类型

Grade VIII

质量水平

表单

lyophilized powder

比活

≥500 units/mg protein

分子量

465 kDa

不包含

BSA as extender

组成

Protein, ≥60% biuret

储存温度

−20°C

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此商品
88597H100918304
grade

for inorganic trace analysis, for ultratrace analysis

grade

-

grade

-

grade

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

solution

form

liquid

form

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

anion traces

bromide (Br-): ≤10 μg/kg, fluoride (F-): ≤1000 μg/kg, phosphate (PO43-): ≤25 μg/kg, chloride (Cl-): ≤25 μg/kg, sulfate (SO42-): ≤50 μg/kg, nitrate (NO3-): ≤250 μg/kg

anion traces

-

anion traces

-

anion traces

-

一般描述

β-半乳糖苷酶是一个四聚体,由四个相同的亚基组成,每个亚基的分子量都为135,000。它是一种含巯基的酶,每个亚基含有19个半胱氨酸残基。[1]

应用

β-半乳糖苷酶可与特异性识别靶分子的抗体偶联(酶免疫测定或EISA)。[2]β-半乳糖苷酶也可用作报告酶以监测启动子的基因表达水平。[3]它可以用作具有抗β-半乳糖苷酶抗体的阳性对照蛋白。

生化/生理作用

β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。

物理属性

四聚体的分子量为465 kDa(每个亚基116.3 kDa)

单位定义

在37 °C,pH 7.3条件下,一个单位每分钟可将1.0μmole邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和D-半乳糖。

外形

含有Tris缓冲盐和氯化镁

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
  • 技术规格说明书

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    Alan Merk et al.
    IUCrJ, 7(Pt 4), 639-643 (2020-07-23)
    We report the determination of the structure of Escherichia coli β-galactosidase at a resolution of ∼1.8 Å using data collected on a 200 kV CRYO ARM microscope equipped with a K3 direct electron detector. The data were collected in a single 24 h
    Assays for bacterial mucin-desulfating sulfatases.
    A M Roberton et al.
    Methods in molecular biology (Clifton, N.J.), 125, 417-426 (2000-05-23)
    K Kato et al.
    Journal of immunology (Baltimore, Md. : 1950), 116(6), 1554-1560 (1976-06-01)
    1. A method for the conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from Escherichia coli is described. The method consists of two main steps: treatment of the Fab' fragments containing sulfhydryl groups with excess N,N'-o-phenylenedimaleimide, to introduce
    Raimond B G Ravelli et al.
    Nature communications, 11(1), 2563-2563 (2020-05-24)
    The increasing demand for cryo-electron microscopy (cryo-EM) reveals drawbacks in current sample preparation protocols, such as sample waste and lack of reproducibility. Here, we present several technical developments that provide efficient sample preparation for cryo-EM studies. Pin printing substantially reduces
    Toshiya Suzuki et al.
    Plant physiology, 173(1), 183-205 (2016-11-12)
    Pollen exine is essential for protection from the environment of the male gametes of seed-producing plants, but its assembly and composition remain poorly understood. We previously characterized Arabidopsis (Arabidopsis thaliana) mutants with abnormal pollen exine structure and morphology that we

    商品

    For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

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