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一般描述
β-半乳糖苷酶是一个四聚体，由四个相同的亚基组成，每个亚基的分子量都为135,000。它是一种含巯基的酶，每个亚基含有19个半胱氨酸残基。
应用
β-半乳糖苷酶可与特异性识别靶分子的抗体偶联（酶免疫测定或EISA）。β-半乳糖苷酶也可用作报告酶以监测启动子的基因表达水平。它可以用作具有抗β-半乳糖苷酶抗体的阳性对照蛋白。
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
物理属性
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
单位定义
在37 °C，pH 7.3条件下，一个单位每分钟可将1.0μmole邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和D-半乳糖。
外形
含有Tris缓冲盐和氯化镁
底物
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Alan Merk et al.
IUCrJ, 7(Pt 4), 639-643 (2020-07-23)
We report the determination of the structure of Escherichia coli β-galactosidase at a resolution of ∼1.8 Å using data collected on a 200 kV CRYO ARM microscope equipped with a K3 direct electron detector. The data were collected in a single 24 h
K Kato et al.
Journal of immunology (Baltimore, Md. : 1950), 116(6), 1554-1560 (1976-06-01)
1. A method for the conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from Escherichia coli is described. The method consists of two main steps: treatment of the Fab' fragments containing sulfhydryl groups with excess N,N'-o-phenylenedimaleimide, to introduce
Raimond B G Ravelli et al.
Nature communications, 11(1), 2563-2563 (2020-05-24)
The increasing demand for cryo-electron microscopy (cryo-EM) reveals drawbacks in current sample preparation protocols, such as sample waste and lack of reproducibility. Here, we present several technical developments that provide efficient sample preparation for cryo-EM studies. Pin printing substantially reduces
Assays for bacterial mucin-desulfating sulfatases.
A M Roberton et al.
Methods in molecular biology (Clifton, N.J.), 125, 417-426 (2000-05-23)
D C Young et al.
Analytical biochemistry, 215(1), 24-30 (1993-11-15)
Bacterial beta-galactosidase is one of several reporter enzymes used in studying the transcriptional activity of eukaryotic promoters. Although it is one of the easiest and least expensive enzymes to assay, its use has been limited because of its low sensitivity
商品
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
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