一般描述
PNGase F（肽N-糖苷酶F)剪切天冬酰胺连接糖蛋白，生成无糖肽链和分离的全长寡糖。
应用
PNGase F（来自米尔伊丽莎白菌）已用于蛋白的去糖基化。
用于使蛋白质脱糖基。
生化/生理作用
如果糖基化天冬酰胺基团在其氨基和羧基端被多肽链取代，则从糖蛋白中切割整个聚糖。
单位定义
一个单元将在37℃，pH7.5下通过SDS-PAGE监测，在1分钟内催化1纳摩尔变性核糖核酸酶B释放N-连接的寡糖。 PNGase F活性的一个 Sigma 单位等于1 IUB毫单位。
外形
以溶于 20 mM Tris HCl (pH 7.5)、50 mM NaCl 和 1 mM EDTA 的溶液形式提供
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
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商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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