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Merck
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G5035

Sigma-Aldrich

粒细胞巨噬细胞集落刺激因子 人

GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

别名:

GM-CSF 人

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About This Item

MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.77

生物来源

human

质量水平

重组

expressed in E. coli

检测方案

≥98% (SDS-PAGE)

形式

lyophilized powder

效能

≤0.1 ng/mL EC50

分子量

14.6 kDa

包装

pkg of 4X5 μg
pkg of 5 μg

储存条件

avoid repeated freeze/thaw cycles

技术

cell culture | mammalian: suitable

杂质

≤1.000 EU/μg

颜色

white

溶解性

water: soluble 0.010 mL, clear, colorless

UniProt登记号

储存温度

−20°C

基因信息

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一般描述

基于蛋白质含量的包装尺寸

生化/生理作用

粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种粒细胞、巨噬细胞和嗜酸性粒细胞谱系中的细胞生长和分化因子。GM-CSF在极低浓度下刺激多潜能祖细胞集落形成,是造血祖细胞在所有分裂过程中存活和增殖的重要因素。 它还能刺激一些上皮细胞和破骨细胞的生长。GM-CSF由多种细胞类型(单核细胞、内皮细胞、T细胞、成纤维细胞、丝裂原刺激的B细胞和脂多糖刺激的巨噬细胞)产生。GM-CSF是一种含有128个氨基酸的单链糖蛋白,采用保守二硫键。 人类和小鼠GM-CSF共享约。序列同源性54%,不发生生物活性交叉反应。

外形

从 0.2μm 过滤的 10mM 柠檬酸钠溶液 (pH3.5) 中冻干。

分析说明

使用人 TF-1 细胞在培养物中检测人 GM-CSF 的增殖活性。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. How many units are present in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?

    The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is less than or equal to 0.1 ng/ml, corresponding to a specific activity of greater than or equal to 1 x 107 units/mg. So the 5 microgram package size (G5035-5UG) should have at least 50,000 units per vial.

  7. There is no visible powder in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human. Is the vial empty?

    This product only contains 5 micrograms of protein without any carrier protein. The product is lyophilized from a small amount of solution so the product may not be visible in the vial.

  8. Can I use Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human, on mouse cells?

    There is only about 56% homology between human and mouse GM-CSF, and no cross-species activity.  We would recommend to use mouse GM-CSF, Product No. G0282.

  9. What is the isoelectric point (pI) for Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?

    The pI is 4.8.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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