G4509
甘油激酶 来源于大肠杆菌
lyophilized powder, 40-100 units/mg protein
别名:
ATP：甘油3-磷酸转移酶, 甘油激酶
产品名称
甘油激酶 来源于大肠杆菌, lyophilized powder, 40-100 units/mg protein
biological source
Escherichia coli
form
lyophilized powder
specific activity
40-100 units/mg protein
composition
Protein, ≥50% biuret
foreign activity
Triose-phosphate isomerase, hexokinase, myokinase, glycerol dehydrogenase and NADH oxidase ≤0.05%
storage temp.
−20°C
Quality Level
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Application
利用甘油激酶（glpK）研究疼痛调控神经肽对人脂肪细胞脂解的影响。
Biochem/physiol Actions
甘油激酶催化甘油的tge MgATP依赖性磷酸化以产生sn-甘油-3-磷酸，并且是甘油利用中的限速酶。 它也受到果糖-1，6-二磷酸的反馈调节。
甘油激酶催化甘油的tge MgATP依赖性磷酸化以产生sn-甘油-3-磷酸，并且是甘油利用中的限速酶。 <<<20,21>>> 它也受到果糖-1，6-二磷酸的反馈调节。<<<22>>>
Other Notes
一个单位为在pH 9.8和25℃下，在PK/LDH的偶联体系内每分钟将1.0 μmole甘油和ATP转化为L-α甘油磷酸盐和ADP。
Physical form
部分纯化的冻干粉末，平衡主要是盐和EDTA。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
F Lönnqvist et al.
Arteriosclerosis, thrombosis, and vascular biology, 17(7), 1472-1480 (1997-07-01)
Cardiovascular complications of obesity are more common in men than women. Sex differences in visceral fat lipolysis may be of importance in this respect, since increased release of free fatty acids (FFAs) from visceral fat to the liver by the
V Van Harmelen et al.
The Journal of biological chemistry, 274(26), 18243-18251 (1999-06-22)
The effects of acylation-stimulating protein (ASP) and insulin on free fatty acid (FFA) release from isolated human fat cells and the signal transduction pathways to induce these effects were studied. ASP and insulin inhibited basal and norepinephrine-induced FFA release by
A Girard et al.
The Biochemical journal, 274 ( Pt 3), 819-824 (1991-03-15)
An artificial-membrane-bound glycerokinase chosen as a membrane-bound two-substrate-enzyme model has been used to separate two unequal compartments of a specially designed diffusion cell. An interesting feature is the asymmetry of compartments and the existence of a diffusion layer adjacent to
S Reynisdottir et al.
Diabetologia, 37(4), 428-435 (1994-04-01)
Upper-body obesity is an important risk factor for developing non-insulin dependent diabetes. To investigate the possibility that a lipolysis defect is present in this form of obesity, we examined the adrenergic regulation of lipolysis in abdominal subcutaneous fat cells from
L Hellström et al.
International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity, 21(4), 314-320 (1997-04-01)
The weight loss achieved during treatment with very-low-calorie diets (VLCD) varies between individuals. The aim of this study was to investigate whether interindividual variations in catecholamine-induced lipolysis are of importance for the rate of weight loss during VLCD. Prospective study.
实验方案
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
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