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Merck
CN

G4388

Sigma-Aldrich

Glycerol 3-phosphate Oxidase from Streptococcus thermophilus

lyophilized powder, ≥35 units/mg solid, pH 7.0

别名:

sn-Glycerol 3-phosphate: oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204

表单

lyophilized powder

比活

≥35 units/mg solid, pH 7.0

分子量

131 kDa

运输

wet ice

储存温度

−20°C

应用

GPO has been used for sensitive metabolite assays of starch and lipid synthesis, pyrophosphate, ATP, ADP, and most glycolytic intermediates in Arabidopsis seeds. GPO is part of the dihydroxyacetone phosphate:glycerol-3-phosphate cycle in the bloodstream form of Trypanosoma brucei.

生化/生理作用

Glycerol-3-phosphate oxidase catalyzes the conversion of sn-glycerol-3-phosphate to glycerone phosphate and peroxide.

单位定义

One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at pH 7.0 at 37 °C.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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分析证书(COA)

Lot/Batch Number

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Localization of glycerol-3-phosphate oxidase in the mitochondrion and particulate NAD+-linked glycerol-3-phosphate dehydrogenase in the microbodies of the bloodstream form to Trypanosoma brucei.
F R Opperdoes et al.
European journal of biochemistry, 76(1), 29-39 (1977-06-01)
Shu-Yi Hsu et al.
Sensors (Basel, Switzerland), 10(6), 5758-5773 (2010-01-01)
A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized
Tatsuya Kishimoto et al.
Clinica chimica acta; international journal of clinical chemistry, 333(1), 59-67 (2003-06-18)
Several methods for measuring concentrations of lysophosphatidic acid (LPA), a lipid mediator, have been reported to date. However, these methods are not routinely used because most of them require specialized instrument and a complicated protocol. We developed a novel LPA
C S Pundir et al.
Clinical biochemistry, 43(4-5), 467-472 (2009-12-29)
A method is described for construction of an amperometric triglyceride (TG) biosensor using PVA membrane bound enzymes. A mixture of commercial lipase, glycerol kinase, glycerol-3-phosphate oxidase and horseradish peroxidase was co-immobilized onto polyvinyl alcohol (PVA) membrane through glutaraldehyde coupling. The
Alain Bizzini et al.
Journal of bacteriology, 192(3), 779-785 (2009-12-08)
Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone

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