G4155
β 半乳糖苷酶 来源于大肠杆菌
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
别名:
β-D-半乳糖苷半乳糖水解酶, 乳糖酶
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一般描述
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
应用
β-半乳糖苷酶用于咪唑并吡咯烷糖的酶促合成。
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
外形
混悬于 50% 甘油、5 mM Tris 缓冲盐、5 mM 氯化镁、0.5 mM DTT、0.5 mM 巯基乙醇中，pH 7.4。
其他说明
在 37°C、pH 7.3 条件下，1 个单元每分钟水解 1.0 μmol 邻硝基苯基 β-D-半乳糖苷，生成邻硝基苯酚和 D-半乳糖。
如果该酶被冷冻，可能发生酶活性丧失。这种酶在 50% 甘油溶液中提供，如果在-20°C 下储存，不会冷冻。不建议将此酶制剂保存在超冷冰箱中 （-60 至-100° C）。
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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Théophile Tschamber et al.
Bioorganic & medicinal chemistry, 11(17), 3559-3568 (2003-08-07)
The syntheses of four glyco-imidazoles, which are pentose-derivatives belonging to the D-series, as well as the syntheses of their L-enantiomers, are reported. Starting from the known linear xylo, lyxo, arabino, and ribo imidazolo-pentoses in both the L- and the D-series
Olga Ermakova et al.
Brain sciences, 11(6) (2021-07-03)
Acquisition of detailed anatomical and molecular knowledge from intact biological samples while preserving their native three-dimensional structure is still a challenging issue for imaging studies aiming to unravel a system's functions. Three-dimensional micro-CT X-ray imaging with a high spatial resolution
A Hinek et al.
The Journal of clinical investigation, 91(3), 1198-1205 (1993-03-01)
We and others have previously shown that a 67-kD cell surface elastin/laminin-binding protein (EBP) is responsible for cell adhesion to elastin and laminin and for mediating the process of elastin fiber assembly, but the nature of this protein was unknown.
E Paschke et al.
Human genetics, 109(2), 159-166 (2001-08-21)
An inherited deficiency in beta-galactosidase can result in GM1 gangliosidosis, with several phenotypes of generalized or chronic psychomotor deterioration, as well as in Morquio disease type B, a characteristic mucopolysaccharidosis free of neurological symptoms. We performed mutation analyses in 17
J W Callahan
Biochimica et biophysica acta, 1455(2-3), 85-103 (1999-11-26)
GM1 gangliosidosis and Morquio B disease are distinct disorders both clinically and biochemically yet they arise from the same beta-galactosidase enzyme deficiency. On the other hand, galactosialidosis and sialidosis share common clinical and biochemical features, yet they arise from two
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