G4155
β 半乳糖苷酶 来源于大肠杆菌
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
别名:
β-D-半乳糖苷半乳糖水解酶, 乳糖酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥500 units/mg protein (biuret)
form
aqueous glycerol suspension
specific activity
≥500 units/mg protein (biuret)
mol wt
465 kDa
storage temp.
−20°C
Quality Level
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General description
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
Application
β-半乳糖苷酶用于咪唑并吡咯烷糖的酶促合成。
Biochem/physiol Actions
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
Physical form
混悬于 50% 甘油、5 mM Tris 缓冲盐、5 mM 氯化镁、0.5 mM DTT、0.5 mM 巯基乙醇中，pH 7.4。
Other Notes
如果该酶被冷冻，可能发生酶活性丧失。这种酶在 50% 甘油溶液中提供，如果在-20°C 下储存，不会冷冻。不建议将此酶制剂保存在超冷冰箱中 （-60 至-100° C）。
在 37°C、pH 7.3 条件下，1 个单元每分钟水解 1.0 μmol 邻硝基苯基 β-D-半乳糖苷，生成邻硝基苯酚和 D-半乳糖。
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
法规信息
常规特殊物品
此项目有
Théophile Tschamber et al.
Bioorganic & medicinal chemistry, 11(17), 3559-3568 (2003-08-07)
The syntheses of four glyco-imidazoles, which are pentose-derivatives belonging to the D-series, as well as the syntheses of their L-enantiomers, are reported. Starting from the known linear xylo, lyxo, arabino, and ribo imidazolo-pentoses in both the L- and the D-series
Olga Ermakova et al.
Brain sciences, 11(6) (2021-07-03)
Acquisition of detailed anatomical and molecular knowledge from intact biological samples while preserving their native three-dimensional structure is still a challenging issue for imaging studies aiming to unravel a system's functions. Three-dimensional micro-CT X-ray imaging with a high spatial resolution
E Paschke et al.
Human genetics, 109(2), 159-166 (2001-08-21)
An inherited deficiency in beta-galactosidase can result in GM1 gangliosidosis, with several phenotypes of generalized or chronic psychomotor deterioration, as well as in Morquio disease type B, a characteristic mucopolysaccharidosis free of neurological symptoms. We performed mutation analyses in 17
A Hinek et al.
The Journal of clinical investigation, 91(3), 1198-1205 (1993-03-01)
We and others have previously shown that a 67-kD cell surface elastin/laminin-binding protein (EBP) is responsible for cell adhesion to elastin and laminin and for mediating the process of elastin fiber assembly, but the nature of this protein was unknown.
J W Callahan
Biochimica et biophysica acta, 1455(2-3), 85-103 (1999-11-26)
GM1 gangliosidosis and Morquio B disease are distinct disorders both clinically and biochemically yet they arise from the same beta-galactosidase enzyme deficiency. On the other hand, galactosialidosis and sialidosis share common clinical and biochemical features, yet they arise from two
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