form
aqueous glycerol suspension
Quality Level
specific activity
≥500 units/mg protein (biuret)
mol wt
465 kDa
storage temp.
−20°C
General description
四聚体的分子量为465 kDa(每个亚基116.3 kDa)
Application
β-半乳糖苷酶用于咪唑并吡咯烷糖的酶促合成。
Biochem/physiol Actions
β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
Physical form
混悬于 50% 甘油、5 mM Tris 缓冲盐、5 mM 氯化镁、0.5 mM DTT、0.5 mM 巯基乙醇中,pH 7.4。
Other Notes
如果该酶被冷冻,可能发生酶活性丧失。这种酶在 50% 甘油溶液中提供,如果在-20°C 下储存,不会冷冻。不建议将此酶制剂保存在超冷冰箱中 (-60 至-100° C)。
在 37°C、pH 7.3 条件下,1 个单元每分钟水解 1.0 μmol 邻硝基苯基 β-D-半乳糖苷,生成邻硝基苯酚和 D-半乳糖。
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
法规信息
常规特殊物品
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G4155-1KU | 04061833630495 |
| G4155-3KU | 04061833630501 |
| G4155-5KU | 04061832843810 |
