G4142
β-Galactosidase from bovine testes
ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)
别名:
β-D-Galactoside galactohydrolase
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
1.0-3.0 units/mg protein (modified Warburg-Christian)
Biological source:
bovine
biological source
bovine
Quality Level
form
ammonium sulfate suspension
specific activity
1.0-3.0 units/mg protein (modified Warburg-Christian)
foreign activity
α- and β-glucosidase ≤0.2%, α-fucosidase ≤0.2%, α-galactosidase ≤0.2%, α-mannosidase ≤0.2%, β-N-acetylglucosaminidase ≤1.0%
storage temp.
2-8°C
Application
β-Galactosidase from bovine testes was used for coupling to Sepharose.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Physical form
Suspension in 3.2 M (NH4)2SO4, pH approx. 5.0
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
W Kiess et al.
The Journal of biological chemistry, 263(19), 9339-9344 (1988-07-05)
Cloning and sequencing of the human type II insulin-like growth factor (IGF) receptor cDNA revealed an 80% deduced amino acid sequence homology with the bovine cation-independent mannose 6-phosphate (Man-6-P) receptor, suggesting identity of the two receptors (Morgan, D. O., Edman
The purification and properties of beta-galactosidase from bovine testes.
J J Distler et al.
The Journal of biological chemistry, 248(19), 6772-6780 (1973-10-10)
D H Juers et al.
Protein science : a publication of the Protein Society, 8(1), 122-136 (1999-04-21)
Beta-galactosidase (lacZ) from Escherichia coli is a 464 kDa homotetramer. Each subunit consists of five domains, the third being an alpha/beta barrel that contains most of the active site residues. A comparison is made between each of the domains and
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G4142-.2UN | 04061832843766 |
| G4142-.5UN | 04061832843773 |