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Merck
CN

G4013

Sigma-Aldrich

谷胱甘肽过氧化物酶 来源于人类红细胞

lyophilized powder, ≥30 units/mg protein

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别名:
GSH-Px, 谷胱甘肽:过氧化氢氧化还原酶
CAS号:
MDL编号:
UNSPSC代码:
12352204

形式

lyophilized powder

比活

≥30 units/mg protein

组成

Protein, 13-25% modified Warburg-Christian

UniProt登记号

运输

dry ice

储存温度

−20°C

基因信息

human ... GPX1(2876)

一般描述

GPX1(谷胱甘肽过氧化物酶 1)属于 GPXs 的四元家族,包括 GPX2、GPX3 和 GPX4。它是一种含硒半胱氨酸的蛋白质。GPX1 是 GPX 酶的主要亚型,广泛存在于细胞内。
注意:在报道的最适 pH 值 8.8 下,我们发现活性约为pH 7.0 时的 10 倍。但是,为了与文献保持一致,避免与 GSH 的非酶催化氧化反应混淆,我们的单位仍在 pH 7.0 下定义。

应用

来自人红细胞的谷胱甘肽过氧化物酶 (GPX) 已被用于测定从皮肤鳞状细胞癌 (CSCC) 或日光性角化病 (SK) 获得的上皮片中过氧化物灭活 GPX 的能力。

生化/生理作用

GPX1(谷胱甘肽过氧化物酶 1)利用还原型谷胱甘肽 (GSH) 作为电子供体,催化多种有机和无机过氧化物的转化。这种酶还负责清除有机和无机过氧化物。

单位定义

在pH7.0,25℃下,一个单元将催化H2O2氧化1.0 μmol的还原型谷胱甘肽至氧化型谷胱甘肽。

外形

含 25% 蔗糖和 3% 二硫苏糖醇以及磷酸钠缓冲盐的冻干粉

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Do you have any solution stability information on glutathione peroxidase from erythrocytes?

    The following information on the bovine source enzyme (equivalent to Product G6137) probably also applies to the human source enzyme (G4013). According to the literature, there is no appreciable loss after 3 months in the presence of 1 mM dithiothreitol (DTT) if solutions of the enzyme are frozen. There is a significant loss (15-22%) in 1 month when in the presence of 2 mM reduced glutathione (GSH), and if neither DTT nor GSH present, the loss is even greater (60-70% lost in 1 month frozen.1There are also stability concerns with the powdered form of Glutathione Peroxidase; the powder is shipped on dry ice and should be kept frozen. We have found that the powder should be stable at least a year if stored in the freezer below 0°C.In our experience, without DTT or mercaptoethanol present, a solution of Glutathione Peroxidase may lose much of its activity within a day, even on ice. Our general recommendation is to reconstitute in 10 mM sodium phosphate, pH 7.0 with at least 1 mM dithiothreitol. The solution should be divided into working aliquots and promptly frozen; it will be stable through one freeze-thaw cycle.1. Biochimica et Biophysica Acta, 358, 251-261 (1974).

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