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生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~18 kDa
种属反应性
human
技术
western blot: 1:500-1:2,000
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... GADD45A(1647)
一般描述
Growth arrest and DNA damage inducible α It is expressed at low levels in testis, but, at moderate level in liver, brain, heart and spleen. GADD45A shows high expression in kidney and skeletal muscle.
免疫原
synthetic peptide corresponding to amino acids 12-25 of human GADD45A
应用
Anti-GADD45A (12-25) antibody produced in rabbit has been used in immunocytochemistry.
Anti-GADD45A antibody produced in rabbit is suitable for western blotting at a working dilution of 1:500-1:2000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
生化/生理作用
GADD45A (growth arrest and DNA-damage-inducible, alpha) gene encodes a protein that belongs to a group of genes that are expressed under stressful growth arrest conditions and upon treatment with DNA-damaging agents such as methylmethane sulfonate and UV radiation. The protein mediates activation of the p38/JNK pathway via MTK1/MEKK4 kinase upon environmental stress. It inhibits entry of cells into S phase by activating DNA excision repair in vitro. It plays a crucial role in cell cycle checkpoints, DNA repair, and genome integrity maintenance.
Growth arrest and DNA damage inducible α (GADD45A) miRNAs are differentially expressed in osteosarcoma. It interacts with proliferating cell nuclear antigen (PCNA), aurora A kinase and histones. GADD45A improves the cytotoxic effect of the drug temozolomide (TMZ) in glioblastoma multiforme (GBM).
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Ossie Geifman-Holtzman et al.
Advances in experimental medicine and biology, 793, 121-129 (2013-10-10)
Preeclampsia is a pregnancy-induced complex of multiple pathological changes. Numerous stresses during pregnancy, including hypoxia, immune activation, inflammatory cytokines, and oxidative stress were reported as contributing factors to the preeclamptic pathology. Seeking common sensors of various stressors in preeclampsia is
GADD45A plays a protective role against temozolomide treatment in glioblastoma cells
Wang HH, et al.
Scientific Reports, 7(1), 8814-8814 (2017)
Jesús M Salvador et al.
Advances in experimental medicine and biology, 793, 1-19 (2013-10-10)
The first identified Gadd45 gene, Gadd45a, encodes a ubiquitously expressed protein that is often induced by DNA damage and other stress signals associated with growth arrest and apoptosis. This protein and the other two members of this small gene family
Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase
Sanchez R, et al.
The Journal of Biological Chemistry, 285(29), 22196-22201 (2010)
Analysis of miRNA-gene expression-genomic profiles reveals complex mechanisms of microRNA deregulation in osteosarcoma
Maire G, et al.
Cancer Genetics, 204(3), 138-146 (2011)
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