G3144
L-谷氨酰胺合成酶 来源于大肠杆菌 W，ATCC 号9637
Protein ≥30 % by biuret, lyophilized powder
别名:
L-谷氨酸:氨连接酶（形成 ADP）
form
lyophilized powder
specific activity
100-400 units/mg protein
composition
Protein, ≥30% biuret
solubility
H2O: soluble 0.95-1.05 mg/mL, clear to slightly hazy
UniProt accession no.
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... glnA(948370)
Application
L-谷氨酰胺合成酶可用于从大肠杆菌中纯化蛋白酶。
Biochem/physiol Actions
谷氨酸降解酶
L-谷氨酰胺合成酶催化L-谷氨酸和氨缩合为L-谷氨酰胺。它是谷氨酸的降解酶。
Physical form
含缓冲盐和稳定剂的冻干粉
Preparation Note
在含葡萄糖和 NH4Cl 的培养基中生长
Other Notes
一个单位将在37℃，pH值7.1下于15分钟内将1.0μ摩尔的L-谷氨酸转化为L-谷氨酰胺。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Jose Luis Ortega et al.
Molecular genetics and genomics : MGG, 287(11-12), 881-893 (2012-10-20)
Glutamine synthetase (GS) catalyzes the synthesis of glutamine from glutamate and ammonia. In plants, it occurs as two major isoforms, a cytosolic form (GS(1)) and a nuclear encoded chloroplastic form. The focus of this paper is to determine the role
Alberto Sola-Landa et al.
Nucleic acids research, 41(3), 1767-1782 (2012-12-19)
Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR-PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator
Lin Xu et al.
Antonie van Leeuwenhoek, 103(3), 559-565 (2012-11-13)
During a study of the diversity and phylogeny of rhizobia isolated from root nodules of Oxytropis ochrocephala grown in the northwest of China, four strains were classified in the genus Rhizobium on the basis of their 16S rRNA gene sequences.
Manuel A Matamoros et al.
The New phytologist, 197(3), 873-885 (2012-12-05)
Legume nodule senescence is a poorly understood process involving a decrease in N(2) fixation and an increase in proteolytic activity. Some physiological changes during nodule aging have been reported, but scarce information is available at the subcellular level. Biochemical, immunological
H S Kingdon et al.
Journal of bacteriology, 94(4), 949-957 (1967-10-01)
The kinetic properties of Escherichia coli glutamine synthetase are markedly influenced by the manner in which the organism is grown. Enzyme obtained from stationary-phase cells grown on glycerol and glutamate is strongely inhibited by each of the eight feedback effectors
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