形式
lyophilized powder
比活
100-400 units/mg protein
组成
Protein, ≥30% biuret
溶解性
H2O: soluble 0.95-1.05 mg/mL, clear to slightly hazy
UniProt登记号
储存温度
−20°C
基因信息
Escherichia coli K12 ... glnA(948370)
应用
L-谷氨酰胺合成酶可用于从大肠杆菌中纯化蛋白酶。
生化/生理作用
L-谷氨酰胺合成酶催化L-谷氨酸和氨缩合为L-谷氨酰胺。它是谷氨酸的降解酶。
谷氨酸降解酶
单位定义
一个单位将在37℃，pH值7.1下于15分钟内将1.0μ摩尔的L-谷氨酸转化为L-谷氨酰胺。
外形
含缓冲盐和稳定剂的冻干粉
制备说明
在含葡萄糖和 NH4Cl 的培养基中生长
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
H S Kingdon et al.
Journal of bacteriology, 94(4), 949-957 (1967-10-01)
The kinetic properties of Escherichia coli glutamine synthetase are markedly influenced by the manner in which the organism is grown. Enzyme obtained from stationary-phase cells grown on glycerol and glutamate is strongely inhibited by each of the eight feedback effectors
S H Liaw et al.
Protein science : a publication of the Protein Society, 4(11), 2358-2365 (1995-11-01)
Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of ammonia and glutamate to yield glutamine, ADP, and inorganic phosphate in the presence of divalent cations. Bacterial GS is an enzyme of 12 identical subunits, arranged in two rings of 6, with
E Rodriguez et al.
Plant physiology and biochemistry : PPB, 62, 19-22 (2012-11-24)
Lead (Pb) is a toxic element, but its putative mutagenic effects in plant cells, using molecular markers, remain to unveil. To evaluate if Pb induces mutagenicity, Pisum sativum L. seedlings were exposed to Pb(2+) (up to 2000 mg L(-1)) for 28 days
Kamal Krishna Singh et al.
Plant cell reports, 32(2), 183-193 (2012-10-17)
KEY MESSAGE : The regulation of GS isoforms by WD was organ specific. Two GS isoforms i.e. OsGS1;1 and OsGS2 were differentially regulated in IR-64 (drought-sensitive) and Khitish (drought-tolerant) cultivars of rice. Water deficit (WD) has adverse effect on rice
Peritumoral hyperplasia of the liver: a response to portal vein invasion by hypervascular neoplasms.
Thomas Arnason et al.
Histopathology, 62(3), 458-464 (2012-12-18)
Several cases of focal nodular hyperplasia (FNH) or similar hyperplastic lesions have been reported adjacent to hepatic neoplasms, including hepatocellular carcinoma, epithelioid haemangioendothelioma and hepatoblastoma. We refer to this hyperplastic response as peritumoral hyperplasia (PTH). Here, we report eight cases
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