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安全信息

G2N10

Sigma-Aldrich

GenElute™ 植物基因组 DNA 微量制备试剂盒

greener alternative

sufficient for 10 purifications

别名:

植物基因组DNA少量制备, Gen Elute

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About This Item

UNSPSC代码:
41105501
NACRES:
NA.55

用途

sufficient for 10 purifications

质量水平

200

环保替代产品特性

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

环保替代产品分类

Aligned,

储存温度

15-25°C

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相关类别

一般描述

GenElute植物基因组DNA小量制备试剂盒可以简单、方便的从各种植物中分离高纯度的DNA。GenElute试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、RNase处理，以及诸如苯酚和氯仿等有害化学成分。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法，本产品符合固有安全化学原则(Inherently Safer Chemistry)。

应用

纯化所得的高纯度DNA可直接供以下敏感的下游应用使用：
  • PCR
  • 限制性内切酶酶切
  • 克隆
  • Southern印迹
GenElute™ Plant Genomic DNA Miniprep Kit has been used:
  • in isolation of arbuscular mycorrhizal (AM) fungal spores DNA
  • in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA
  • in genomic DNA isolation from leaves

特点和优势

  • 起始材料：不超过100 mg的植物组织
  • 预期得率：至多20 μg
  • 洗脱体积：100 - 200 μl
  • 所需时间：<40分钟
  • 是否需要RNase处理：否

原理

在1小时内，可从至多100 mg新鲜组织或10 mg冻干材料中提取出微克级的DNA。首先在液氮中研磨植物组织，在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作，去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤，进一步纯化基因组DNA。纯化所得DNA长度超过20 kb。

其他说明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • C2112Column Preparation Solution化学品安全说明书

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

补充剂危害

EUH032

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Peter Kämpfer et al.
Systematic and applied microbiology, 35(1), 19-23 (2011-12-16)
A bright yellow pigmented bacterium was isolated from the leaf surface of Trifolium repens in Germany. Comparative analysis of 16S rRNA gene sequences showed that this bacterium is most closely related to Duganella zoogloeoides IAM 12670(T), with a similarity of
Carolina Martín et al.
PloS one, 6(8), e23979-e23979 (2011-09-09)
Increasing germplasm erosion requires the recovery and conservation of traditional cultivars before they disappear. Here we present a particular case in Spain where a thorough prospection of local fruit tree species was performed in the 1950s with detailed data of
Tobias Janke et al.
Current microbiology, 67(2), 156-169 (2013-03-12)
Conventional microbiological techniques yield only limited information on the composition of fungal communities in dust. The aim of this study was to establish and optimize PCR-single strand conformation polymorphism (PCR-SSCP) analysis for investigation of fungal diversity in rural dust samples.
Tae-Jin Kang et al.
BMC biotechnology, 4, 20-20 (2004-09-03)
DNA extraction methods for PCR-quality DNA from calluses and plants are not time efficient, since they require that the tissues be ground in liquid nitrogen, followed by precipitation of the DNA pellet in ethanol, washing and drying the pellet, etc.
Santoshkumar M Shetty et al.
Journal of plant physiology, 169(7), 718-730 (2012-03-02)
The transcriptional regulation of multigenic eggplant (Solanum melongena) polyphenol oxidase genes (SmePPO) is orchestrated by their corresponding promoters which mediate developmentally regulated expression in response to myriad biotic and abiotic factors. However, information on structural features of SmePPO promoters and
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实验方案

GenElute™ 植物基因组DNA Miniprep试剂盒实验方案(G2N10, G2N70, G2N350)

本方案描述了使用GenElute植物基因组DNA Miniprep试剂盒，从多种植物中分离纯DNA的简单、便捷操作步骤。

GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

Plant DNA Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

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