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一般描述
来自链霉菌属的L-谷氨酸氧化酶（LGOX）在麦麸预培养基中细胞外分泌。它对应于120 kDa的分子量和61 kDa的单体亚基。LGOX的结构由六聚体组成，并具有黄素腺嘌呤二核苷酸辅基。
该产物具有带有凝血酶切割位点的6x组氨酸标签。
应用
来自链霉菌属的L-谷氨酸氧化酶可用作：
- 水凝胶聚（氨基甲酰基磺酸盐）固定化生物传感器的参考酶
- 作为铅笔石墨电极的组成部分用于生物传感L-谷氨酸
生化/生理作用
L-谷氨酸氧化酶（LGOX）催化L-谷氨酸氧化脱氨为α-酮戊二酸、氨和过氧化氢。LGOX是工业生产的，主要用作L-谷氨酸检测的生物传感器和L-谷氨酸试剂盒。
单位定义
一个单位为在pH 7.4，30℃下，每分钟从L-谷氨酸形成1.0微摩尔的α-酮戊二酸。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Isolation, purification, and characterization of L-glutamate oxidase from Streptomyces sp. 18G
Wachiratianchai S, et al.
Electronic journal of Biotechnology, 7(3), 09-10 (2004)
L-Glutamate biosensor based on l-glutamate oxidase immobilized onto ZnO nanorods/polypyrrole modified pencil graphite electrode
Batra B, et al.
Biochemical Engineering Journal, 105, 428-436 (2016)
Comparative study of hydrogel-immobilized L-glutamate oxidases for a novel thick-film biosensor and its application in food samples
Kwong AWK, et al.
Biotechnology Letters, 22(4), 267-272 (2000)
Jiro Arima et al.
The FEBS journal, 276(14), 3894-3903 (2009-06-18)
L-Glutamate oxidase (LGOX) from Streptomyces sp. X-119-6, which catalyzes the oxidative deamination of L-glutamate, has attracted increasing attention as a component of amperometric L-glutamate sensors used in the food industry and clinical biochemistry. The precursor of LGOX, which has a
Andrea C Kneuttinger et al.
Cell chemical biology, 26(11), 1501-1514 (2019-09-10)
Imidazole glycerol phosphate synthase (ImGPS) is an allosteric bienzyme complex in which substrate binding to the synthase subunit HisF stimulates the glutaminase subunit HisH. To control this stimulation with light, we have incorporated the photo-responsive unnatural amino acids phenylalanine-4'-azobenzene (AzoF)
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