推荐产品
等级
for molecular biology
质量水平
形式
liquid
包装
vial of 1 mL
浓度
19-22 mg/mL
储存温度
−20°C
InChI
1S/C24H42O21/c25-1-5-9(28)11(30)16(35)22(41-5)39-4-8-20(45-23-17(36)12(31)10(29)6(2-26)42-23)14(33)18(37)24(43-8)44-19-7(3-27)40-21(38)15(34)13(19)32/h5-38H,1-4H2/t5-,6-,7-,8-,9-,10-,11+,12+,13-,14-,15-,16-,17-,18-,19-,20-,21+,22+,23-,24-/m1/s1
InChI key
BYSGBSNPRWKUQH-UJDJLXLFSA-N
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一般描述
Glycogen is a branched polymer of glucose synthesized by animal cells for energy storage and release. It is constructed of predominantly α1→4 glycosidic bonds with branches created through α1→6 glycosidic bonds.
Glycogen is a branched polymer of glucose that coprecipitates nucleic acids during isolation and purification.
应用
Glycogen from Mytilus genus (Blue mussel) has been used
- as a coprecipitant in the isolation of RNA from hepatocytes and yeast cells.
- as a reference standard in calibration curve generation to quantify hepatic glycogen content.
- as a carrier molecule for DNA and RNA, replacing tRNA or sonicated DNA
- for the precipitation of DNA in the presence of ammonium acetate and ethanol or isopropanol
- for recovery of tRNA, yeast RNA, sonicated DNA and oligonucleotides as short as 8 base pairs
生化/生理作用
Glycogen levels in Mytilus genus shows variation during seasonal changes. It is a crucial stored energy reserve for Mussels and is utilized during gametogenesis. Glycogen levels dictate the reproductive capacity and development of mussels larva. Glycogen aids in the coprecipitation of nucleic acids during isolation and purification.
特点和优势
- product provided is 1 mL total volume
- precipitates even small amounts of DNA and RNA present in dilute solutions
- does not interfere with OD 260/280 readings
原理
Glycogen is an inert carrier that traps nucleic acids. It forms a precipitate with DNA/RNA while being insoluble in ethanol solution. Due to the manufacturing process, there is low risk of contaminating nucleic acids in the glycogen. This product is tested for DNase, RNase, and NICKase, but not nucleic acids.
相关产品
产品编号
说明
价格
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Regulation of gluconeogenesis by Kruppel-like factor 15
Gray S, et al.
Cell Metabolism, 5(4), 305-312 (2007)
Energy storage and reproduction in mussels, Mytilus galloprovincialis: the influence of diet quality
Fearman JA, et al.
Journal of shellfish research., 28(2), 305-312 (2009)
Biomass composition of blue mussels, Mytilus edulis, is affected by living site and species of ingested microalgae
Pleissner D, et al.
ISRN zoology, 2012 (2012)
Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells
Cruz C, et al.
eLife, 7, e34081-e34081 (2018)
Ryan J Farr et al.
PLoS pathogens, 17(7), e1009759-e1009759 (2021-07-29)
The host response to SARS-CoV-2 infection provide insights into both viral pathogenesis and patient management. The host-encoded microRNA (miRNA) response to SARS-CoV-2 infection, however, remains poorly defined. Here we profiled circulating miRNAs from ten COVID-19 patients sampled longitudinally and ten
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