G1549
PNGase F Glycosidase
de-glycosylates N-linked glycoproteins for analysis, suitable for mass spectrometry, recmobinant, expressed in E. coli
别名:
糖苷酶F 来源于脑膜脓毒性伊丽莎白菌, N-糖苷酶F, 糖苷酶F 来源于脑膜炎脓毒性黄杆菌, 糖苷酶F 来源于脑膜脓毒性金黄杆菌, 肽N-糖苷酶
应用
用于使蛋白质去糖基化。
重组PNGase F经亲和色谱纯化后，透析到pH 7.5、含10 mM磷酸钾的50%甘油溶液中，得到稳定的产物。该产品含有少量缓冲盐。这种高度纯化的材料可用于制备性去糖基化或用于分析凝胶、溶液或印迹膜。可利用该酶C端的6x组氨酸融合标签而将其从制备性操作中去除。来源于Elizabethkingia meningoseptica的PNGase F可用于人血浆样品的去糖基化分析和硫酸软骨素蛋白聚糖的去糖基化。
生化/生理作用
从糖蛋白切割整个聚糖，前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。
来源于Elizabethkingia meningoseptica的PNGase F在N端具有聚糖结合催化结构域和碗状结构域。如果糖基化天冬酰胺部分在其氨基和羧基端被多肽链取代，则它可从糖蛋白中切割整个聚糖。它在原核宿主中经济有效地大规模生产，需要二价锌离子才能发挥其酶促活性。
外形
是浓度为300单位酶/mL的50%（v/v）甘油和50%（v/v）20 mM磷酸钾溶液（pH 7.5）。
其他说明
一个酶活性单位是指在37℃、pH 7.5条件下通过SDS-PAGE监测，在1分钟内催化1nM变性核糖核酸酶B释放N-连接寡糖所需的酶量。糖苷酶F活性的一个Sigma单位相当于1 IUB毫单位。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
Discovery and characterization of a novel extremely acidic bacterial N-glycanase with combined advantages of PNGase F and A
Wang T, et al.
Bioscience Reports, 34(6), e00149-e00149 (2014)
Characterization and analysis of extracellular matrix in malignant brain tumors and their cellular derivatives
Extracellular Matrix, 113-138 (2015)
Identification and characterization of a novel prokaryotic peptide: N-glycosidase from Elizabethkingia meningoseptica
Sun G, et al.
The Journal of Biological Chemistry, jbc-M114 (2015)
N-glycosylation of apolipoprotein A1 in cardiovascular diseases
Majek P, et al.
Translational Research, 165(2), 360-362 (2015)
Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome.
Klara Pecankova et al.
PloS one, 17(1), e0262484-e0262484 (2022-01-11)
Extracellular vesicles are released into body fluids from the majority of, if not all, cell types. Because their secretion and specific cargo (e.g., proteins) varies according to pathology, extracellular vesicles may prove a rich source of biomarkers. However, their biological
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).
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