生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates
western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
绿色荧光蛋白（GFP）生色团含具有Phe64-Ser-Tyr-Gly-Val-Gln69氨基酸序列的六肽片段。GFP结构包含11链β-桶状结构和中心螺旋，对应分子量为26 kDa。
特异性
对于GFP融合蛋白有特异性。
免疫原
合成多肽对应GFP氨基酸3-17。
应用
兔抗GFP（N末端抗体）抗体用于：
- 蛋白质印迹
- 组织化学和免疫组化
- 免疫沉淀
生化/生理作用
绿色荧光蛋白（GFP）应用于光化学和诊断领域。也广泛应用于蛋白动力学研究。多克隆抗-GFP、兔抗N端抗体可与GFP融合蛋白发生特异性反应。
外形
溶于0.01M磷酸盐缓冲盐水，pH 7.4，含有1%牛血清白蛋白以及15 mM叠氮化钠。
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
含少量动物源组分生物产品
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