G1163
O-糖苷酶 来源于肺炎链球菌
recombinant, expressed in E. coli, buffered aqueous solution
别名:
α-内切乙酰氨基半乳糖苷酶, O-糖酵素
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
232-888-2
NACRES:
NA.32
MDL number:
Recombinant:
expressed in E. coli
Concentration:
≥800 units/mL
recombinant
expressed in E. coli
conjugate
(O-linked)
form
buffered aqueous solution
mol wt
180 kDa
concentration
≥800 units/mL
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Biochem/physiol Actions
从糖蛋白上释放未取代的Ser-和Thr-连接β-Gal-(1→3)-α-GalNAc（核心1型O-聚糖)。 用唾液酸、乳糖胺（半乳糖-N-乙酰氨基葡萄糖）或岩藻糖取代的二糖核心将阻止水解并防止寡糖从蛋白质中释放出来。在使用O-糖苷酶切割之前，可能需要使用糖酵解酶进行预处理以从O-聚糖中去除取代糖。
Packaging
提供5×反应缓冲液，250mM NaH2PO4，pH 5.0。
Physical form
溶于50 mM磷酸钾，pH 7.5
Analysis Note
筛查是否存在：β-半乳糖苷酶、α-甘露糖苷酶、β-己糖胺酶、α-岩藻糖苷酶、神经氨酸酶和蛋白酶。有关特定批次的信息，请参阅分析证书。
Other Notes
在37 °C、pH 6.5下，一单位在每分钟内可水解1 μmole的对硝基苯基半乳糖N-生物苷（β-Gal-(1→3)-α-GalNAc-1→ΟC6H4NO2）。
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
I Brockhausen
Biochimica et biophysica acta, 1473(1), 67-95 (1999-12-02)
Glycoproteins with O-glycosidically linked carbohydrate chains of complex structures and functions are found in secretions and on the cell surfaces of cancer cells. The structures of O-glycans are often unusual or abnormal in cancer, and greatly contribute to the phenotype
Roudabeh J Jamasbi et al.
Hybridoma and hybridomics, 22(6), 367-376 (2003-12-20)
A mouse monoclonal antibody (MAb-9) produced by immunization with a human esophageal carcinoma cell line, TE-2 (derived from undifferentiated squamous cell carcinoma) reacted specifically with about 30% of esophageal carcinoma cell lines and tissue sections from clinical samples. MAb-9 showed
Nelia A Tobey et al.
Digestive diseases and sciences, 55(7), 1856-1865 (2010-05-27)
The structures that contribute to shunt resistance (Rs) in esophageal epithelium are incompletely understood, with 35-40% of Rs known to be calcium-dependent, reflecting the role of e-cadherin. Two calcium-independent candidates for the remaining approximately 60% of Rs have been identified:
B S Dunbar et al.
Biology of reproduction, 65(3), 951-960 (2001-08-22)
A lactosaminoglycan-associated antigen is associated with a carbohydrate moiety of all three zona pellucida (ZP) glycoproteins of pig and rabbit but is absent in the mouse and rat. A monoclonal antibody (PS1) recognizing this determinant was obtained by immunizing mice
H Yamini Shrivastava et al.
Journal of biomolecular structure & dynamics, 21(5), 671-680 (2004-02-11)
In the present study, the impact of chromium(III) complexes ([Cr(salen)(H2O)2](+) (1), [Cr(en)3]3+ (2) and [Cr(EDTA)(H2O)]- (3)) on the biophysical properties of mucin like specific viscosity, zeta potential and particle size has been investigated. It is evident from the present investigation
商品
Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.
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