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安全信息

G1163

Sigma-Aldrich

O-糖苷酶 来源于肺炎链球菌

recombinant, expressed in E. coli, buffered aqueous solution

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别名:
α-内切乙酰氨基半乳糖苷酶, O-糖酵素
CAS号:
9032-92-2
EC 号:
3.2.1.97 (BRENDA, IUBMB)
EC 号:
232-888-2
MDL编号:
MFCD00131003
UNSPSC代码:
12352204
NACRES:
NA.32

重组

expressed in E. coli

质量水平

200

偶联物

(O-linked)

形式

buffered aqueous solution

分子量

180 kDa

浓度

≥800 units/mL

运输

wet ice

储存温度

2-8°C

相关类别

生化/生理作用

从糖蛋白上释放未取代的Ser-和Thr-连接β-Gal-(1→3)-α-GalNAc（核心1型O-聚糖)。 用唾液酸、乳糖胺（半乳糖-N-乙酰氨基葡萄糖）或岩藻糖取代的二糖核心将阻止水解并防止寡糖从蛋白质中释放出来。在使用O-糖苷酶切割之前，可能需要使用糖酵解酶进行预处理以从O-聚糖中去除取代糖。

包装

提供5×反应缓冲液，250mM NaH2PO4，pH 5.0。

单位定义

在37 °C、pH 6.5下，一单位在每分钟内可水解1 μmole的对硝基苯基半乳糖N-生物苷（β-Gal-(1→3)-α-GalNAc-1→ΟC6H4NO2）。

外形

溶于50 mM磷酸钾，pH 7.5

分析说明

筛查是否存在：β-半乳糖苷酶、α-甘露糖苷酶、β-己糖胺酶、α-岩藻糖苷酶、神经氨酸酶和蛋白酶。有关特定批次的信息，请参阅分析证书。

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

分析证书(COA)

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