产品名称
Sephadex® G-10, Medium
technique(s)
protein array: suitable
matrix active group
phase
swelling
1 g swells to 2-3 mL
application(s)
life science and biopharma
compatibility
Cytiva
Quality Level
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Application
Sephadex® G-10可作为体积排阻材料用于将标记的N-聚糖与多余的染料分离。还可用于脱盐以降低盐浓度用于阴离子交换(AEx)和阳离子交换色谱(CEx)。
Sephadex® G-10已被用于将膜蛋白重建为巨大的蛋白脂质体。
Biochem/physiol Actions
Sephadex G-10是凝胶过滤介质，用于凝胶过滤色谱和蛋白质色谱。应用广泛，已被用于癌症研究，并可用于测定自来水、河水和绿茶等多种样品中的氟化物含量。
Features and Benefits
- 一步完成脱盐、除污染和置换新缓冲液。
- 适合用于缓冲液置换和生物样品纯化。
- 适合分离分子量超过700的多肽、小分子蛋白和多糖。
- 高效去除盐、染料和放射性标记。
- 具有Sephadex G型中最低的700分子量排阻极限
General description
Sephadex G-10葡聚糖凝胶是常见的凝胶过滤树脂，专门设计用于多肽和生物小分子的脱盐和缓冲液置换。该型号具有Sephadex中最小的排阻极限，令其在这些应用中十分有效。Sephadex是葡聚糖与环氧氯丙烷交联制备而成的凝胶过滤树脂。不同类型的Sephadex的交联度不同，因此其溶胀程度和分子分级范围也不同。Sephadex G-10属于G型，G型Sephadex的型号包括从G-10（用于小分子）到G-75（用于大分子）的5种型号。
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
N-glycan analysis by CGE-LIF: profiling influenza A virus hemagglutinin N-glycosylation during vaccine production
Schwarzer J, et al.
Electrophoresis (2008)
Kaushal Rege et al.
Nature protocols, 5(3), 408-417 (2010-03-06)
Methods development in chromatography is a time-consuming, trial-and-error process that requires laborious experimentation. We describe a high-throughput screening (HTS) protocol for the rapid identification of chromatographic steps for protein purification from cell-free expression broths. Broths containing the protein are loaded
Jinlan Zhang et al.
Journal of food protection, 72(12), 2524-2529 (2009-12-17)
Pentocin 31-1, an anti-Listeria bacteriocin produced by Lactobacillus pentosus 31-1 from the traditional Chinese fermented Xuan-Wei ham, was successfully purified by the pH-mediated cell adsorption-desorption method and then purified by gel chromatography with Sephadex G-10. The purification resulted in a
Kwang Wook Hyun et al.
Peptides, 27(6), 1173-1178 (2005-11-18)
This study describes the extraction and characterization of a platelet aggregation inhibitory peptide from Inonotus obliquus. Ethanol extract from I. obliquus ASI 74006 mycelia showed the highest platelet aggregation inhibitory activity (81.2%). The maximum platelet aggregation inhibitory activity was found
M Muirhead et al.
International journal of cell cloning, 8(6), 392-408 (1990-11-01)
We have cloned a stromal cell from mouse bone marrow selected on the basis of its ability to promote the growth of an Abelson virus-transformed pre-B cell line. These stromal cells have smooth muscle features, and the ultrafiltrate of stromal
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