G0535
糖肽酶 A 来源于杏仁
buffered aqueous glycerol solution, ≥0.05 unit/mL
别名:
N-糖苷酶A, N-连接糖肽-(N-乙酰基-β-D-氨基葡萄糖)-L-天冬酰胺酰胺水解酶, 糖酰胺酶A
产品名称
糖肽酶 A 来源于杏仁, buffered aqueous glycerol solution, ≥0.05 unit/mL
conjugate
(N-linked)
form
buffered aqueous glycerol solution
concentration
≥0.05 unit/mL
storage temp.
−20°C
Quality Level
Application
来自杏仁的糖肽酶 A 用于去糖基化。它催化去除 N-连接的寡糖链并将 Asn 残基转化为 Asp。
Biochem/physiol Actions
水解 N4-(乙酰基-D-葡糖胺基)天冬酰胺，其中 N-乙酰基-D-葡糖胺残基可进一步糖基化，产生（取代的）N-乙酰基-β-D-葡糖胺基胺和含有天冬氨酸残基的肽。
General description
杏仁中发现的糖肽酶可分为 A、B 和 C 三组。糖肽酶 A 的最适 pH 值和等电点分别为 6.0 和 7.7。它偏爱长链糖肽。它还能够水解完整的糖蛋白，例如去唾液酸化的人转铁蛋白、卵清蛋白等。这些蛋白质在它们的 β-天冬氨酰-葡糖基胺键处切割具有去唾液酸碳水化合物部分的糖蛋白。
Other Notes
在 pH 5.0、37°C 条件下，1 个单位将每分钟水解1.0 μmole 卵清蛋白糖肽。
Physical form
含 50 mM 柠檬酸盐-磷酸盐缓冲液（pH 5.0）和 BSA 的 50% 甘油溶液。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
法规信息
新产品
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Biochimica et biophysica acta, 657(2), 457-467 (1981-02-13)
The glycopeptidase preparation that has been isolated from almond emulsin and acts on beta-aspartylglycosylamine linkages in glycopeptides was separated into three active fractions by DEAE-cellulose column chromatography. The three discrete species of glycopeptidase (Groups A, B and C) have been
Asparagine-linked oligosaccharides in human placenta and umbilical cord as demonstrated by almond glycopeptidase.
N Takahashi et al.
FEBS letters, 146(1), 139-142 (1982-09-06)
Karen G Welinder et al.
The Journal of biological chemistry, 284(15), 9764-9769 (2009-02-13)
Proteome data of potato (Solanum tuberosum) tuber juice and of purified potato tuber vacuoles indicated that mature patatins may perhaps lack a C-terminal propeptide. We have confirmed this by complete mass spectrometric sequencing of a number of patatin variants as
R P Miller et al.
Biochimica et biophysica acta, 954(1), 50-57 (1988-04-28)
The beta-subunit of dog kidney (Na+ + K+)-ATPase is a sialoglycoprotein and contains three potential N-glycosylation sites. In this study, the oligosaccharide chains of purified dog kidney beta-subunit were labeled with tritium by oxidation with sodium periodate or galactose oxidase
A plant peptide: N-glycanase orthologue facilitates glycoprotein ER-associated degradation in yeast.
Yuki Masahara-Negishi et al.
Biochimica et biophysica acta, 1820(10), 1457-1462 (2012-06-05)
The cytoplasmic peptide:N-glycanase (PNGase) is a deglycosylating enzyme involved in the ER-associated degradation (ERAD) process, while ERAD-independent activities are also reported. Previous biochemical analyses indicated that the cytoplasmic PNGase orthologue in Arabidopsis thaliana (AtPNG1) can function as not only PNGase
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Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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