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一般描述
杏仁中发现的糖肽酶可分为 A、B 和 C 三组。糖肽酶 A 的最适 pH 值和等电点分别为 6.0 和 7.7。它偏爱长链糖肽。它还能够水解完整的糖蛋白，例如去唾液酸化的人转铁蛋白、卵清蛋白等。这些蛋白质在它们的 β-天冬氨酰-葡糖基胺键处切割具有去唾液酸碳水化合物部分的糖蛋白。
应用
来自杏仁的糖肽酶 A 用于去糖基化。它催化去除 N-连接的寡糖链并将 Asn 残基转化为 Asp。
生化/生理作用
水解 N4-(乙酰基-D-葡糖胺基)天冬酰胺，其中 N-乙酰基-D-葡糖胺残基可进一步糖基化，产生（取代的）N-乙酰基-β-D-葡糖胺基胺和含有天冬氨酸残基的肽。
单位定义
在 pH 5.0、37°C 条件下，1 个单位将每分钟水解1.0 μmole 卵清蛋白糖肽。
外形
含 50 mM 柠檬酸盐-磷酸盐缓冲液（pH 5.0）和 BSA 的 50% 甘油溶液。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
No data available
闪点(°C)
No data available
法规信息
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Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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