G0282
粒细胞巨噬细胞集落刺激因子 来源于小鼠
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
别名:
GM-CSF
产品名称
粒细胞巨噬细胞集落刺激因子 来源于小鼠, GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
mouse
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg)
quality
endotoxin tested
mol wt
~15 kDa (125 amino acids including N-terminal methionine)
packaging
pkg of 5 and 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
color
white
solubility
water: soluble, clear, colorless
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
mouse ... CSF2(12981), Csf2(12981)
Analysis Note
使用小鼠 FDP-1 细胞在培养物中检测了小鼠 GM-CSF 的 EC50 活性。
Biochem/physiol Actions
粒细胞-巨噬细胞集落刺激因子（GM-CSF）是粒细胞、巨噬细胞和嗜酸性粒细胞系的生长和分化因子。GM-CSF 在极低浓度下刺激多能祖细胞集落形成，是造血祖细胞直至成熟的所有分裂中必不可少的存活和增殖因子。它也刺激一些上皮细胞和破骨细胞的生长。GM-CSF 由多种细胞类型（单核细胞、内皮细胞、T 细胞、成纤维细胞、有丝分裂原刺激的 B 细胞和 LPS 刺激的巨噬细胞）产生。GM-CSF 是一种单链糖蛋白，含有 128 个氨基酸，具有保守的二硫键。人和鼠 GM-CSF 的序列同源性约为 54%，生物活性无交叉反应。
Physical form
从 10 mM 乙酸 + 250 μg BSA 中冻干。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
A Kelso
Immunology and cell biology, 76(4), 300-317 (1998-09-02)
Cytokines participate in the induction and effector phases of all immune and inflammatory responses. They are therefore obvious tools and targets for strategies designed to promote, inhibit or redirect these responses. However, the complexity of the cytokine network has hindered
Specificity of signaling by hematopoietic cytokine receptors: instructive versus permissive effects.
R C Skoda
Journal of receptor and signal transduction research, 19(1-4), 741-772 (1999-03-11)
The helical cytokines constitute a family of proteins with a common three-dimensional structure. They exert a wide variety of biological effects with a preference for the hematopoietic system. The effects of helical cytokines are mediated by cell surface receptors, which
M A Guthridge et al.
Stem cells (Dayton, Ohio), 16(5), 301-313 (1998-10-10)
The process of ligand binding leading to receptor activation is an ordered and sequential one. High-affinity binding of GM-CSF, interleukin 3 (IL-3), and IL-5 to their receptors induces a number of key events at the cell surface and within the
M H Heim
Journal of receptor and signal transduction research, 19(1-4), 75-120 (1999-03-11)
The Jak-STAT pathway was originally discovered through the study of interferon induced intracellular signal transduction. Meanwhile, a large number of cytokines, hormones and growth factors have been found to activate Jaks and STATs. Jaks (Janus Kinases) are a unique class
Vasiliki Kyrargyri et al.
Glia, 63(4), 549-566 (2014-10-10)
Microglia are CNS resident immune cells and a rich source of neuroactive mediators, but their contribution to physiological brain processes such as synaptic plasticity, learning, and memory is not fully understood. In this study, we used mice with partial depletion
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